LacZ in pLX304
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42560||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Growth in Bacteria
Growth Strain(s)NEB Stable
Insert Size (bp)2792
- Promoter CMV
/ Fusion Protein
- 2xV5 (C terminal on insert)
- Cloning method Gateway Cloning
- 5′ sequencing primer pLXF
- 3′ sequencing primer pLXR (Common Sequencing Primers)
Please note that there are a few minor sequence discrepancies between the provided depositor's sequence and Addgene quality control sequence, most notably the 6xHis tag is missing.
These changes should not affect function and this plasmid can still be used as control.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LacZ in pLX304 was a gift from William Hahn (Addgene plasmid # 42560 ; http://n2t.net/addgene:42560 ; RRID:Addgene_42560)
For your References section:beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex for Survival and Tumorigenesis. Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI, Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, Goessling W, Hahn WC. Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. Epub 2012 Dec 13. 10.1016/j.cell.2012.11.026 PubMed 23245941