CX-nestinGFPas
(Plasmid #32401)

• Depositing Labs: Patrick Salmon, Henryk Zulewski
• Publication: Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;345(3):1167-76. Epub 2006 May 11.

Backbone

• Vector backbone: CX lentiviral vector
• Modifications to backbone: insertion of the whole nestin promoter-GFP-nestin enhancer and betaglobin polyA into a CX lentiviral vector
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nestin promoter/enhancer GFP
• Insert Size (bp): 5900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (destroyed during cloning)
• 3′ cloning site: NotI (destroyed during cloning)
• 5′ sequencing primer: gacagagacagatccattc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that there are some discrepancies between Addgene's QC sequence and the depositor's assembled sequence. The plasmid should still function as reported.