Purpose(Empty Backbone) SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: hygromycin resistance gene
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60524||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 2021
Vector typeMammalian Expression ; Transposon
- Promoter EF1a/RPBSA
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer GCCTCAGACAGTGGTTCAAAG
- 3′ sequencing primer AGGCACAGTCGAGGCTGAT (Common Sequencing Primers)
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Note that an SfiI digest will affect the Kozak sequence that is already present upstream of the insertion site for the gene of interest. This can be addressed by adding the 3 bases 'ACC' between the 5'-SfiI site and the start codon of your gene of interest:
5'-SfiI---> ggcctctgaggcc ACC atg <---start codon
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSBbi-Hyg was a gift from Eric Kowarz (Addgene plasmid # 60524 ; http://n2t.net/addgene:60524 ; RRID:Addgene_60524)
For your References section:Optimized Sleeping Beauty transposons rapidly generate stable transgenic cell lines. Kowarz E, Loescher D, Marschalek R. Biotechnol J. 2015 Feb 4. doi: 10.1002/biot.201400821. 10.1002/biot.201400821 PubMed 25650551