|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||17817||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Bacterial Resistance(s)Tetracycline, 10 μg/mL
Copy numberLow Copy
Gene/Insert namemevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
SpeciesS. cerevisiae (budding yeast); E. coli
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
does not contain lacI/
ERG1, ERG8, MVD1: S. cerevisiae/
idi, ispA: E. Coli
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMBIS was a gift from Jay Keasling (Addgene plasmid # 17817 ; http://n2t.net/addgene:17817 ; RRID:Addgene_17817)
For your References section:Engineering a mevalonate pathway in Escherichia coli for production of terpenoids. Martin VJ, Pitera DJ, Withers ST, Newman JD, Keasling JD. Nat Biotechnol. 2003 Jul . 21(7):796-802. 10.1038/nbt833 PubMed 12778056