166,461 results

1. pBPGUw

   Plasmid

   #17575

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Tools for neuroanatomy and neurogenetics

   Insert

   GAL4 (GAL4 Budding Yeast)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. human beta-catenin pcDNA3

   Plasmid

   #16828

   Purpose

   Mammalian expression of human beta-catenin with Flag tag

   Depositor

   Eric Fearon

   Article

   Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. MS2-epegRNA-Dnmt1

   Plasmid

   #211372

   Purpose

   Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MS2-epegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF Con/Fon DREADD Gq-mCherry

   Plasmid

   #183532

   Purpose

   INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre AND Flp

   Depositor

   Karl Deisseroth

   Insert

   DREADD Gq-mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   None

   Promoter

   nEF

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. V406 4HA-CKIe(K38R)

   Plasmid

   #13725

   Depositor

   David Virshup

   Article

   Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

   Insert

   casein kinase 1 epsilon (CSNK1E Human)
   

   Use

   Tags

   4 HA

   Expression

   Mammalian

   Mutation

   kinase-inactive due to K38R mutation

   Promoter

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. YAP/TAZ tdTomato TEAD Binding Reporter

   Plasmid

   #228554

   Purpose

   Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

   Depositor

   Cole DeForest

   Article

   Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

   Insert

   tdTomato
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GTIIC TEAD-Binding Responsive Element

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBbA5c-MevT(CO)-MBIS(CO, ispA)

   Plasmid

   #35151

   Depositor

   Jay Keasling, Taek Soon Lee

   Article

   Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

   Insert

   AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   R364S in Mevalonate Kinase

   Promoter

   LacUV5,

   Available Since

   July 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPB-TetON-mEGFP-MYOD1_AroPERFECT

   Plasmid

   #215620

   Purpose

   For integration of MYOD1 AroPERFECT T2A mEGFP

   Depositor

   Denes Hnisz

   Article

   Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

   Insert

   MYOD1 AroPERFECT (MYOD1 Human)
   

   Use

   Tags

   T2A mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-Ezrin-N-14

   Plasmid

   #55043

   Purpose

   Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Ezrin (EZR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    William Hahn, David Root, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. STK11 gRNA (BRDN0001146194)

    Plasmid

    #75913

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human STK11

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    STK11 (STK11 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMAL-c2X

    Plasmid

    #75286

    Purpose

    Expresses proteins in the cytoplasm as fusions to maltose-binding protein

    Depositor

    Paul Riggs

    Article

    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1

    Type

    Empty backbone

    Use

    Tags

    maltose-binding protein

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 18, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. Fuw-dCas9-Tet1CD-P2A-BFP

    Plasmid

    #108245

    Purpose

    Lentiviral vector to express dCas9-Tet1CD-P2A-tagBFP

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

    Insert

    dCas9-Tet1CD-HA-P2A-BFP
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    dCas9-D10A, dCas9-H840A

    Promoter

    Ubc

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-EF1a-HA-ATAD3A-IRES-Hygro

    Plasmid

    #211469

    Purpose

    Lentivirus backbone expressing ATAD3A with an HA-tag at the N-terminal

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ATAD3A (ATAD3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pON.mCherry

    Plasmid

    #84821

    Purpose

    Constitutive Expression plasmid with mCherry fluorescent protein

    Depositor

    Howard Shuman

    Article

    Gebhardt et al PLoS One. 2017 Mar 3;12(3):e0173116. doi

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tac Promoter

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAS9-mCherry-Frame +2

    Plasmid

    #66941

    Purpose

    CRISPaint frame selector +2

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    gRNA frame +2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Ef1a-DIO ChETA-EYFP

    Plasmid

    #26968

    Purpose

    AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    hChR2(E123T/H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123T, H134R

    Promoter

    EF1alpha

    Available Since

    Jan. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDB179

    Plasmid

    #91960

    Purpose

    empty expression vector for 10His-SUMO tag protein purification

    Depositor

    Christopher Bahl

    Type

    Empty backbone

    Use

    Tags

    10-His and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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