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pBABE puro PPAR gamma 2
(Plasmid #8859)


Item Catalog # Description Quantity Price (USD)
Plasmid 8859 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    Available at Addgene (#1764)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    PPAR gamma
  • Alt name
    PPAR gamma 2
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    Pparg (a.k.a. Nr1c3, PPAR-gamma, PPAR-gamma2, PPARgamma, PPARgamma2)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The PPARg2 cDNA was isolated from the adipocyte lambda ZAPII library derived from pBluescript-PPARg2 plasmid (Tontonoz et al 1994, Genes Dev 8:1224-1234) as an XbaI-XhoI fragment. Blunt end ligation into EcoRI site of pBABE puro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE puro PPAR gamma 2 was a gift from Bruce Spiegelman (Addgene plasmid # 8859 ; ; RRID:Addgene_8859)
  • For your References section:

    Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Tontonoz P, Hu E, Spiegelman BM. Cell. 1994 Dec 30. 79(7):1147-56. 10.1016/0092-8674(94)90006-X PubMed 8001151