pBabe bleo human PPAR gamma 2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11439||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBabe-zeo (aka: pBabe-bleo)
Backbone manufacturerpBabe-zeo is available at Addgene
- Backbone size w/o insert (bp) 5169
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert namePPAR gamma 2
SpeciesH. sapiens (human)
Insert Size (bp)1525
Entrez GenePPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (destroyed during cloning)
- 3′ cloning site SalI (destroyed during cloning)
- 5′ sequencing primer pBABE 5
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Human PPARg2 (-10 to 1515 TAG) - insert was cut with XhoI and cloned into vector cut with SalI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBabe bleo human PPAR gamma 2 was a gift from Ronald Kahn (Addgene plasmid # 11439 ; http://n2t.net/addgene:11439 ; RRID:Addgene_11439)