Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24143||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerGreen lab
- Backbone size w/o insert (bp) 4874
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameIkB alpha
SpeciesH. sapiens (human)
Entrez GeneNFKBIA (a.k.a. EDAID2, IKBA, MAD-3, NFKBI)
/ Fusion Protein
- 3xHA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer hGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PCMV4-3 HA/IkB-alpha(SS32,36AA) was a gift from Warner Greene (Addgene plasmid # 24143 ; http://n2t.net/addgene:24143 ; RRID:Addgene_24143)
For your References section:Both amino- and carboxyl-terminal sequences within I kappa B alpha regulate its inducible degradation. Sun S, Elwood J, Greene WC. Mol Cell Biol. 1996 Mar . 16(3):1058-65. PubMed 8622650