PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46914||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSICO derivative
- Backbone size w/o insert (bp) 8200
- Total vector size (bp) 8308
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Copy numberLow Copy
Insert Size (bp)103
- Promoter U6
- Cloning method Restriction Enzyme
- 5′ cloning site BstX1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer cttgtgggagaagctcggcta (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pU6-sgGFP-NT1 was a gift from Stanley Qi & Jonathan Weissman (Addgene plasmid # 46914 ; http://n2t.net/addgene:46914 ; RRID:Addgene_46914)
For your References section:CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes. Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044. 10.1016/j.cell.2013.06.044 PubMed 23849981