cdk1 FRET sensor (2327)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||26064||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameCdk1 FRET Sensor
/ Fusion Proteins
- YFP variant (N terminal on insert)
- CFP variant (C terminal on insert)
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Original plasmid: pEYFP-C1 from Clontech, CMV promoter has been modified to introduce a T7 sequence and contains the extra sites HindIII, SacI and NcoI.
Domains: YFP variant-PoloBox Domain-flexible linker-Phospho site-CFP variant-FLAG
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:cdk1 FRET sensor (2327) was a gift from Jonathon Pines (Addgene plasmid # 26064 ; http://n2t.net/addgene:26064 ; RRID:Addgene_26064)
For your References section:Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Gavet O, Pines J. Dev Cell. 2010 Apr 20. 18(4):533-43. 10.1016/j.devcel.2010.02.013 PubMed 20412769