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cdk1 FRET sensor (2327)
(Plasmid #26064)


Item Catalog # Description Quantity Price (USD)
Plasmid 26064 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Cdk1 FRET Sensor
  • Tags / Fusion Proteins
    • YFP variant (N terminal on insert)
    • CFP variant (C terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Original plasmid: pEYFP-C1 from Clontech, CMV promoter has been modified to introduce a T7 sequence and contains the extra sites HindIII, SacI and NcoI.

Domains: YFP variant-PoloBox Domain-flexible linker-Phospho site-CFP variant-FLAG

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cdk1 FRET sensor (2327) was a gift from Jonathon Pines (Addgene plasmid # 26064 ; ; RRID:Addgene_26064)
  • For your References section:

    Progressive activation of CyclinB1-Cdk1 coordinates entry to mitosis. Gavet O, Pines J. Dev Cell. 2010 Apr 20. 18(4):533-43. 10.1016/j.devcel.2010.02.013 PubMed 20412769