|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61840||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneCDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please note that this construct may have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-cdc2 (716) was a gift from Jonathon Pines (Addgene plasmid # 61840 ; http://n2t.net/addgene:61840 ; RRID:Addgene_61840)
For your References section:MPF localization is controlled by nuclear export. Hagting A, Karlsson C, Clute P, Jackman M, Pines J. EMBO J. 1998 Jul 15;17(14):4127-38. 10.1093/emboj/17.14.4127 PubMed 9670027