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pcDNA3-cdc2 (716)
(Plasmid #61840)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61840 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cdc2
  • Alt name
    CDK1
  • Species
    H. sapiens (human)
  • Entrez Gene
    CDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGHrev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note that this construct may have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-cdc2 (716) was a gift from Jonathon Pines (Addgene plasmid # 61840 ; http://n2t.net/addgene:61840 ; RRID:Addgene_61840)
  • For your References section:

    MPF localization is controlled by nuclear export. Hagting A, Karlsson C, Clute P, Jackman M, Pines J. EMBO J. 1998 Jul 15;17(14):4127-38. 10.1093/emboj/17.14.4127 PubMed 9670027