pcDNA3-cdc2 (716)
(Plasmid #61840)

• Purpose: mammalian expression of cdc2
• Depositing Lab: Jonathon Pines
• Publication: Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cdc2
• Alt name: CDK1
• Species: H. sapiens (human)
• Entrez Gene: CDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGHrev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this construct may have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.