|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39872||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesH. sapiens (human)
MutationAF = T14A, Y15F
Entrez GeneCDK1 (a.k.a. CDC2, CDC28A, P34CDC2)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Phosphorylation site mutant of cdc2/Cdk1 that cannot be down-regulated by Wee1. Please note that this construct may also have a few mismatches from the available sequence for the CDK1 UTR. These changes do not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-cdc2-AF (718) was a gift from Jonathon Pines (Addgene plasmid # 39872 ; http://n2t.net/addgene:39872 ; RRID:Addgene_39872)
For your References section:MPF localization is controlled by nuclear export. Hagting A, Karlsson C, Clute P, Jackman M, Pines J. EMBO J. 1998 Jul 15;17(14):4127-38. 10.1093/emboj/17.14.4127 PubMed 9670027