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Addgene

pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
(Plasmid #40731)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40731 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pIRES-puro2b
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5125
  • Total vector size (bp) 7062
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Aurora B FRET-sensor
  • Alt name
    CyPet-FHA2-Aurora B substrate-YPet
  • Alt name
    cytoplasmic Aurora B biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    1937
  • Promoter CMV
  • Tags / Fusion Proteins
    • CyPet (N terminal on insert)
    • YPet (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Michael Lampson, Department of Biology, University of Pennsylvania, Philadelphia, US

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b was a gift from Daniel Gerlich (Addgene plasmid # 40731 ; http://n2t.net/addgene:40731 ; RRID:Addgene_40731)
  • For your References section:

    Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW. J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16. 10.1083/jcb.201112112 PubMed 22801782