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pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b
(Plasmid #40571)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40571 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRES-puro2b
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5125
  • Total vector size (bp) 7504
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Aurora B FRET-sensor N-terminally fused to histone 2B
  • Alt name
    H2B-CyPet-FHA2-Aurora B substrate-YPet
  • Alt name
    chromatin-targeted Aurora B biosensor
  • Species
    Synthetic
  • Insert Size (bp)
    2379
  • Promoter CMV
  • Tags / Fusion Proteins
    • CyPet (N terminal on insert)
    • YPet (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BsrGI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Michael Lampson, Department of Biology, University of Pennsylvania, Philadelphia, US
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b was a gift from Daniel Gerlich (Addgene plasmid # 40571 ; http://n2t.net/addgene:40571 ; RRID:Addgene_40571)
  • For your References section:

    Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. Wurzenberger C, Held M, Lampson MA, Poser I, Hyman AA, Gerlich DW. J Cell Biol. 2012 Jul 23;198(2):173-83. Epub 2012 Jul 16. 10.1083/jcb.201112112 PubMed 22801782