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Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.
Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
50835GANLS-DEVD-BNESExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
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50836BNLSExpresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).
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50840RANLS-DEVD-BNESExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
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50842GANES-DEVD-BNLSExpresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS.
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50843RANLSExpresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.
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50849NESRA-DEVD-BNLSExpresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS.
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50852GANLSExpresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.
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60883single polypeptide FPX biosensor for caspase-3FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
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60884single polypeptide FPX biosensor for caspase-8FPX biosensor for caspase-8
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60885single polypeptide FPX biosensor for caspase-1FPX biosensor for caspase-1
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60886single polypeptide FPX biosensor for both caspase-3 and -8FPX biosensor
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60887single polypeptide FPX biosensor for calcium ionFPX biosensor
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60936pPHT-PKAFluorescent FPX sensor for cAMP
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60937pPHT-PIP2Fluorescent FPX sensor for PIP2
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60972RANLS-IETD-BNESExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
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60973RANLS-LEHD-BNESExpresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
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60974Ratiometric FPX sensor for ERK kinase activityFor imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES.
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61017GB-NESddGFP B-NES
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61018GA-NESddGFP A-NES
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61019RA-NESddRFP A-NES
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61020GA-DEVD-GB-NESgreen ddFP based caspase-3 biosensor
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