Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange.
Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. PubMed Article
Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE
Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 50835 | GANLS-DEVD-BNES | Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS). |
| 50836 | BNLS | Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES). |
| 50840 | RANLS-DEVD-BNES | Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS). |
| 50842 | GANES-DEVD-BNLS | Expresses a tandem green ddFP heterodimer in mammalian cells, construct 1 for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid RANLS. |
| 50843 | RANLS | Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS. |
| 50849 | NESRA-DEVD-BNLS | Expresses a tandem red ddFP heterodimer in mammalian cells, construct 1 for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with a second plasmid GANLS. |
| 50852 | GANLS | Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS. |
| 60883 | single polypeptide FPX biosensor for caspase-3 | FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs). |
| 60884 | single polypeptide FPX biosensor for caspase-8 | FPX biosensor for caspase-8 |
| 60885 | single polypeptide FPX biosensor for caspase-1 | FPX biosensor for caspase-1 |
| 60886 | single polypeptide FPX biosensor for both caspase-3 and -8 | FPX biosensor |
| 60887 | single polypeptide FPX biosensor for calcium ion | FPX biosensor |
| 60936 | pPHT-PKA | Fluorescent FPX sensor for cAMP |
| 60937 | pPHT-PIP2 | Fluorescent FPX sensor for PIP2 |
| 60972 | RANLS-IETD-BNES | Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS). |
| 60973 | RANLS-LEHD-BNES | Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS). |
| 60974 | Ratiometric FPX sensor for ERK kinase activity | For imaging of ERK kinase activity using a single polypeptide RA-WW domain-ERK substrate-B protein and free GA-NES. |
| 61017 | GB-NES | ddGFP B-NES |
| 61018 | GA-NES | ddGFP A-NES |
| 61019 | RA-NES | ddRFP A-NES |
| 61020 | GA-DEVD-GB-NES | green ddFP based caspase-3 biosensor |