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single polypeptide FPX biosensor for calcium ion
(Plasmid #60887)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60887 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3 (+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 8278
  • Modifications to backbone
    multi-cloning sites have been modified to put 5' XhoI and 3' HindIII sites in the backbone
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    single polypeptide FPX biosensor for calcium ion
  • Alt name
    ddRFP A-Calmodulin-ddFP B-M13-ddGFP A
  • Alt name
    dimerization dependent red or green fluorescent protein (ddRFP or ddGFP) copy A and copy B
  • Species
    Synthetic; synthetic construct
  • Insert Size (bp)
    2778
  • GenBank ID
    KM891585
  • Promoter CMV
  • Tag / Fusion Protein
    • HindIII site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    single polypeptide FPX biosensor for calcium ion was a gift from Robert Campbell (Addgene plasmid # 60887)
  • For your References section:

    Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. 10.1038/nmeth.3261 PubMed 25622108