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Ratiometric FPX sensor for ERK kinase activity
(Plasmid #60974)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 60974 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7493
  • Modifications to backbone
    Multi cloning sites has been revised
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ddRFP A-WW domain-ERK substrate-ddFP B
  • Alt name
    RA-WW domain-ERK substrate-B
  • Species
    Synthetic; synthetic construct
  • Insert Size (bp)
    1993
  • GenBank ID
    KM979350
  • Promoter CMV
  • Tags / Fusion Proteins
    • WW domain is after ddRFP-A
    • ERK substrate is before ddFP copy B
    • ddFP copy B is after ERK substrate

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7 Promoter forward primer
  • 3′ sequencing primer BGH Reverse primers
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ratiometric FPX sensor for ERK kinase activity was a gift from Robert Campbell (Addgene plasmid # 60974)
  • For your References section:

    Ratiometric biosensors based on dimerization-dependent fluorescent protein exchange. Ding Y, Li J, Enterina JR, Shen Y, Zhang I, Tewson PH, Mo GC, Zhang J, Quinn AM, Hughes TE, Maysinger D, Alford SC, Zhang Y, Campbell RE. Nat Methods. 2015 Jan 26. doi: 10.1038/nmeth.3261. 10.1038/nmeth.3261 PubMed 25622108