High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs.
Huang TP, Heins ZJ, Miller SM, Wong BG, Balivada PA, Wang T, Khalil AS, Liu DR
Nat Biotechnol. 2022 Sep 8. pii: 10.1038/s41587-022-01410-2. doi: 10.1038/s41587-022-01410-2. PubMed Article
Huang TP, Heins ZJ, Miller SM, Wong BG, Balivada PA, Wang T, Khalil AS, Liu DR
Nat Biotechnol. 2022 Sep 8. pii: 10.1038/s41587-022-01410-2. doi: 10.1038/s41587-022-01410-2. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183679 | pCMV-eNme2-C-BE4max | Expresses eNme2-C-BE4 in mammalian cells |
| 185667 | pCMV-eNme2-C-ABE8e | Expresses eNme2-C-ABE8e in mammalian cells |
| 185668 | pCMV-eNme2-T.1-ABE8e | Expresses eNme2-T.1-ABE8e in mammalian cells |
| 185669 | pCMV-eNme2-T.2-ABE8e | Expresses eNme2-T.2-ABE8e in mammalian cells |
| 185670 | pCMV-SpRY-HF1-ABE8e | Expresses SpRY-HF1-ABE8e in mammalian cells |
| 185671 | pCMV-SpRY-ABE8e | Expresses SpRY-ABE8e in mammalian cells |
| 185672 | pCMV-eNme2-C.NR | Expresses nuclease active eNme2-C.NR in mammalian cells |
| 185724 | pTPH412 | Directed evolution of Cas9 adenine base editors with altered PAM compatibility |
| 185725 | pTPH418b | Directed evolution of Cas9 adenine base editors with altered PAM compatibility |
| 185726 | pTPH405c | Directed evolution of Cas9 adenine base editors with altered PAM compatibility |
| 185728 | pTPH413 | Bacterial PAM profiling of adenine base editor variants |