pCMV-eNme2-T.2-ABE8e
(Plasmid
#185669)
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PurposeExpresses eNme2-T.2-ABE8e in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV with a BR322 origin
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameecTadA(8e)-neNme2-T.2
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Insert Size (bp)3834
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MutationNme2Cas9 D16A/E47K/R63K/V68M/A116T/T123A/D152N/E154K/E221D/T396A/H452R/E460K/N674S/D720A/A724S/K769R/S816I/D844A/E932K/K940R/M951R/K1005R/D1028N/S1029A/R1033N/R1049C/L1075M
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-eNme2-T.2-ABE8e was a gift from David Liu (Addgene plasmid # 185669 ; http://n2t.net/addgene:185669 ; RRID:Addgene_185669) -
For your References section:
High-throughput continuous evolution of compact Cas9 variants targeting single-nucleotide-pyrimidine PAMs. Huang TP, Heins ZJ, Miller SM, Wong BG, Balivada PA, Wang T, Khalil AS, Liu DR. Nat Biotechnol. 2022 Sep 8. pii: 10.1038/s41587-022-01410-2. doi: 10.1038/s41587-022-01410-2. 10.1038/s41587-022-01410-2 PubMed 36076084
