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GolgiCFP
(Plasmid #14873)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 14873 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CFP
  • Tag / Fusion Protein
    • eNOS(1-33) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Sequences encoding the amino-terminal 33 residues of endothelial nitric-oxide synthase were fused in frame to the 5'-end of CFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GolgiCFP was a gift from Alexandra Newton (Addgene plasmid # 14873 ; http://n2t.net/addgene:14873 ; RRID:Addgene_14873)
  • For your References section:

    Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. Gallegos LL, Kunkel MT, Newton AC. J Biol Chem. 2006 Oct 13. 281(41):30947-56. 10.1074/jbc.M603741200 PubMed 16901905