|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||14873||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- eNOS(1-35) (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
Sequences encoding the amino-terminal 35 residues of endothelial nitric-oxide synthase were fused in frame to the 5'-end of CFP.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GolgiCFP was a gift from Alexandra Newton (Addgene plasmid # 14873 ; http://n2t.net/addgene:14873 ; RRID:Addgene_14873)
For your References section:Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling. Gallegos LL, Kunkel MT, Newton AC. J Biol Chem. 2006 Oct 13. 281(41):30947-56. 10.1074/jbc.M603741200 PubMed 16901905