|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||12570||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerModified from Clontech's pMSCV
- Backbone size (bp) 6800
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
/ Fusion Protein
- TAP (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer MSCV primer (Common Sequencing Primers)
A cDNA encoding the TAP tag was PCR-cloned from the plasmid pBSactTAP with the following primers: left primer 5' -GGACAATG ATCAGGCACCATGGCAGGCCTTGCGCAACACG-3' and right primer 5' -CCGGAATTCCCGGCGGCCGCGGGATCCATTCGCTCGAGGGAC CACCACCACCACCAAGTGCCCCGGAGGATGAG-3' . The PCR product was then cut with BclI/EcoRI and inserted into pMSCV-puro (Clontech) cut with BglII/EcoRI. See author's map for more information (Note that the NotI site is not precisely underlined in the author's map).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMSCV-TAP was a gift from David Sabatini (Addgene plasmid # 12570 ; http://n2t.net/addgene:12570 ; RRID:Addgene_12570)
For your References section:mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458