Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, Luban J
Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 10.1016/j.cell.2020.09.032. PubMed Article
Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, Luban J
Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 10.1016/j.cell.2020.09.032. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158074 | pcDNA3.1 SARS-CoV-2 S D614 | Expresses SARS-CoV-2 spike protein |
| 158075 | pcDNA3.1 SARS-CoV-2 S D614G | Expresses SARS-CoV-2 spike protein with a D614G mutation |
| 158076 | pcDNA3.1 SARS-CoV-2 S D614-trunc | Expresses SARS-CoV-2 spike protein with a truncation |
| 158077 | pcDNA3.1 SARS-CoV-2 S D614G-trunc | Expresses SARS-CoV-2 spike protein with a D614G truncated mutation |
| 158078 | pcDNA3.1 SARS-CoV-2 M | Expresses SARS-CoV-2 membrane protein in mammalian cells |
| 158079 | pcDNA3.1 SARS-CoV-2 N | Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells |
| 158080 | pcDNA3.1 SARS-CoV-2 E | Expresses SARS-CoV-2 envelope protein in mammalian cells |
| 158081 | pscALPSpuro-HsACE2 (human) | Expresses human ACE2 in mammalian cells |
| 158082 | pscALPSpuro-FcACE2 (Cat) | Expresses cat ACE2 in mammalian cells |
| 158083 | pscALPSpuro-ClACE2 (Dog) | Expresses dog ACE2 in mammalian cells |
| 158084 | pscALPSpuro-MjACE2 (Pangolin) | Expresses pangolin ACE2 in mammalian cells |
| 158085 | pscALPSpuro-SsACE2 (Pig) | Expresses pig ACE2 in mammalian cells |
| 158086 | pscALPSpuro-RnACE2 (Rat) | Expresses rat ACE2 in mammalian cells |
| 158087 | pscALPSpuro-MmACE2 (Mouse) | Expresses mouse ACE2 in mammalian cells |
| 158088 | pscALPSblasti-TMPRSS2 Blasti | Expresses TMPRSS2 in mammalian cells |
| 158089 | pCAGGS-ACE2-his expression plasmid | Expresses ACE2 in mammalian cells |
| 159665 | pscALPSpuro-RsACE2 | Expresses Rhinolophus sinicus ACE2 in mammalian cells |
| 164651 | pCAGGS D614G soluble Spike | Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation |
| 164652 | pCAGGS D614 soluble Spike | Expresses SARS-CoV-2 soluble Spike protein |