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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,023 results for: golden gate

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Showing: 4041 - 4060 of 5023 results

  1. pRS479

    Plasmid
    #250493
    Purpose
    Regulatable CRISPRi plasmid for use in Candida albicans. Contains dCas9-Mxi1 controlled by a tetO promoter, NEUT5L integration site and the sgRNA cloning site
    Depositor
    Rebecca Shapiro
    Article
    Wensing et al Nat Microbiol. 2026 May 11. doi: 10.1038
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    250493-plasmid-map-sequence-id-499651

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  2. pYSD741

    Plasmid
    #253052
    Purpose
    Encodes MFapp8 Sig Pep, Hydrophobin1 (HFB1), MYC-TEV-649-stalk surface display anchor in a yeast expression vector with pTEF2 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS origin
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Yeast
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253052-plasmid-map-sequence-id-508946

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  3. pYSD704

    Plasmid
    #253013
    Purpose
    Encodes Trichoderma reesei Hydrophobin1 (HFB1) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Bacterial
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253013-plasmid-map-sequence-id-508761

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  6. pYSD344

    Plasmid
    #253044
    Purpose
    Encodes 2xFLAG-tagged Aga2 with Protein A ZZ domain as a C-terminal fusion in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2 Protein A
    Expression
    Yeast
    Available Since
    May 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    253044-plasmid-map-sequence-id-508939

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  7. pYSD090

    Plasmid
    #252980
    Purpose
    Encodes MYC-tagged 649 stalk display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MYC-tagged 649 stalk display anchor
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252980-plasmid-map-sequence-id-508739

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  8. pYSD089

    Plasmid
    #252979
    Purpose
    Encodes 2xFLAG-tagged 649 stalk display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged 649 stalk display anchor
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252979-plasmid-map-sequence-id-508738

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  9. pYSD078

    Plasmid
    #252975
    Purpose
    Encodes a MoClo Yeast Toolkit (YTK) Type 3 GFP dropout part that can be used to make a YTK GFP dropout vector containing all part types except Type 3
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Protein A
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252975-plasmid-map-sequence-id-508688

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  10. pYSD890

    Plasmid
    #253016
    Purpose
    Encodes S. cerevisiae Met15 as a Type 6 part to be used in the MoClo yeast toolkit (YTK) and yeast secretion/display (YSD2.0) extension of the MoClo YTK
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    MET15
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    253016-plasmid-map-sequence-id-508685

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  11. pYSD086

    Plasmid
    #252976
    Purpose
    Encodes FLAG-tagged S. cerevisiae Sed1p display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged Sed1
    Expression
    Bacterial
    Available Since
    May 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    252976-plasmid-map-sequence-id-508653

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  14. pYSD056

    Plasmid
    #252969
    Purpose
    Encodes S. cerevisiae HA Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252969-plasmid-map-sequence-id-507371

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  15. pYSD057

    Plasmid
    #252970
    Purpose
    Encodes S. cerevisiae 2xFLAG-Tag-Aga2-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252970-plasmid-map-sequence-id-507372

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  16. pYSD054

    Plasmid
    #252967
    Purpose
    Encodes S. cerevisiae MYC Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252967-plasmid-map-sequence-id-507369

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  17. pYSD055

    Plasmid
    #252968
    Purpose
    Encodes S. cerevisiae Aga2-(NoTag)-linker as a Type 3a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252968-plasmid-map-sequence-id-507370

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  18. pYSD053

    Plasmid
    #252966
    Purpose
    Encodes S. cerevisiae 2xFLAG-Tag-Aga2-linker as a Type 3a' part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit to create fusions of proteins of interest to the C-terminus of Aga2
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252966-plasmid-map-sequence-id-507367

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  19. pYSD088

    Plasmid
    #252978
    Purpose
    Encodes 2xFLAG-Tagged S. cerevisiae Aga2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD2.0) MoClo toolkit for N-terminal fusion
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    FLAG-tagged Aga2
    Expression
    Bacterial
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    252978-plasmid-map-sequence-id-507358

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  20. pYSD281

    Plasmid
    #253022
    Purpose
    Encodes the Suc2 signal peptide, Invertase, C-terminal GFP11-M3 and 6xHistidine tags in a yeast expression vector with pTEF1 promoter, LEU2 selectable marker
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Suc2
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253022-plasmid-map-sequence-id-507352

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Showing: 4041 - 4060 of 5023 results