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We narrowed to 5,282 results for: golden gate

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Showing: 4041 - 4060 of 5282 results

  1. L4866 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF1a in MGEV

    Plasmid
    #244190
    Purpose
    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF1a
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF1 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244190-plasmid-map-sequence-id-489496

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  2. L4864 IL-10Rb NTEVp chain, IL-10Ra CTEVp chain with ZF6a in MGEV

    Plasmid
    #244189
    Purpose
    Multigene vector for constitutive expression of mTagBFP2, IL-10Rb NTEVp chain with human IgG VH signal peptide, and IL-10Ra CTEVp chain with ZF6a
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mTagBFP2; IL-10Rb NTEVp chain with human IgG VH signal sequence; IL-10Ra CTEVp chain with ZF6 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244189-plasmid-map-sequence-id-489495

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  7. L4385 dsRedExpress2 reporter, IL-10Rb NTEVp, IL-10Ra CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244186
    Purpose
    PiggyBac transposon vector for expression of dsRedExpress2 under synTF promoter; constitutive expression of IL-10Rb NTEVp chain, IL-10Ra CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRedExpress2 under synTF responsive promoter; IL-10Rb NTEVp chain with human CD8a SS; IL-10Ra CTEVp chain; mNeonGreen-P2A-PuroR (CD8A Human, Synthetic)
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244186-plasmid-map-sequence-id-489485

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  15. pRDB_031

    Plasmid
    #245331
    Purpose
    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63
    Depositor
    John Doench, David Root
    Insert
    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245331-plasmid-map-sequence-id-488051

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  16. pMV_AA100

    Plasmid
    #245322
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD55
    Depositor
    John Doench, David Root
    Insert
    Cas9, sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245322-plasmid-map-sequence-id-488050

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  17. pRDB_246

    Plasmid
    #245333
    Purpose
    Cas12a CRISPRko positive control guide; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245333-plasmid-map-sequence-id-488048

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  18. pRDA_908

    Plasmid
    #245330
    Purpose
    Cas12a CRISPRa positive control guide; targets CD274, ADGRE5, CD4, DPP4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274, ADGRE5, CD4, DPP4 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245330-plasmid-map-sequence-id-488046

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  19. pRDB_245

    Plasmid
    #245332
    Purpose
    Cas12a CRISPRko positive control guide; targets CD46
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245332-plasmid-map-sequence-id-488043

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  20. pRDB_512

    Plasmid
    #245338
    Purpose
    Cas9 ABE & CBE positive control guide; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245338-plasmid-map-sequence-id-488042

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Showing: 4041 - 4060 of 5282 results