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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,193 results for: golden gate

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Showing: 4041 - 4060 of 5193 results

  1. SMT503

    Plasmid
    #226840
    Purpose
    Plasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. Chloramp
    Depositor
    Polly Fordyce
    Article
    Thompson et al Adv Mater Interfaces. 2025 Feb 3;12(3):2
    Insert
    Lipase A
    Use
    Synthetic Biology
    Tags
    SNAP and eGFP
    Expression
    Bacterial
    Mutation
    R33Q, D34N, K35D, K112D, M134D, Y139C, I157M
    Promoter
    T7
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    226840-plasmid-map-sequence-id-456035

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  2. pVL1710

    Plasmid
    #239543
    Purpose
    Express Yea4p membrane protein cargo fused to C-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    ScYea4p-2xCrk_SH3-mTurq2-TEVsite-Venus
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239543-plasmid-map-sequence-id-476623

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  3. pKS1614

    Plasmid
    #239545
    Purpose
    Express truncated VoGES, Idi1, and Erg20WW for synthesis of geraniol in the peroxisome with Venus as peroxisomal marker
    Depositor
    John Dueber
    Insert
    tVoGES-ePTS1
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239545-plasmid-map-sequence-id-479032

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  4. pVL1708

    Plasmid
    #239542
    Purpose
    Express Yea4p membrane protein cargo fused to N-terminal Venus and TEV site, GAL-inducible TEV protease, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    Venus-TEVsite-ScYea4p-mTurq2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239542-plasmid-map-sequence-id-476621

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  9. pKS1603

    Plasmid
    #239527
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239527-plasmid-map-sequence-id-479035

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  10. pPSG804

    Plasmid
    #239534
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YDR514C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YDR514C
    Use
    CRISPR
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239534-plasmid-map-sequence-id-479034

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  11. pVL1173

    Plasmid
    #239536
    Purpose
    Express CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239536-plasmid-map-sequence-id-479033

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  12. pKS1616

    Plasmid
    #239547
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239547-plasmid-map-sequence-id-479031

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  13. pN4

    Plasmid
    #233521
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1-10
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233521-plasmid-map-sequence-id-471179

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  14. pSEVAb827

    Plasmid
    #217500
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217500-plasmid-map-sequence-id-455513

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  15. pSEVAb837

    Plasmid
    #217501
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217501-plasmid-map-sequence-id-444290

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  16. pSEVAb847

    Plasmid
    #217502
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217502-plasmid-map-sequence-id-444279

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  17. pSEVAb867

    Plasmid
    #217503
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217503-plasmid-map-sequence-id-444286

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  18. pSEVAb887

    Plasmid
    #217504
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217504-plasmid-map-sequence-id-444288

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  19. pSEVAb897

    Plasmid
    #217505
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori
    Depositor
    Nico Claassens
    Article
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available Since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217505-plasmid-map-sequence-id-444281

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Showing: 4041 - 4060 of 5193 results