We narrowed to 21,722 results for: pET

1. pET tdTomato LIC cloning vector (u-tdTomato)

   Plasmid

   #86926

   Purpose

   pET LIC cloning vector for yORF-tdTomato

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Tags

   tdTomato

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET mCitrine LIC cloning vector (u-mCitrine)

   Plasmid

   #29771

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   mCitrine

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET mCerulean LIC cloning vector (u-mCerulean)

   Plasmid

   #29773

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   mCerulean

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET PPL His6 LIC cloning vector (2J-T)

   Plasmid

   #37182

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6 and PPL

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-TEV-Aurein1.2_(+36GFP)-LPETG-His

   Plasmid

   #71755

   Purpose

   His-TEV-Aurein1.2+36GFP-LPETG-His expression in E.Coli

   Depositor

   David Liu

   Article

   Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

   Insert

   Aurein1.2_(pos36_GFP)-LPETG-His
   

   Tags

   6XHis, Sortase (LPETG), and TEV Protease

   Expression

   Bacterial

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

   Plasmid

   #171587

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS 6His

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   MKIEE-NLS-RfxCas13d-NLS-HA-6His
   

   Tags

   HA-6HIS and MKIEE

   Expression

   Bacterial

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b(+)_SARS-CoV-2_3CLpro-C145A-Q306A

   Plasmid

   #177335

   Purpose

   bacterial expression of inactive SARS-CoV-2 3C-like-C145A proteinase

   Depositor

   Christopher Overall

   Article

   Pablos et al Cell Rep. 2021 Oct 26;37(4):109892. doi:

   Insert

   SARS-CoV-2 3C-like (C145A) proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2) (ORF1ab SARS-CoV-2 virus)
   

   Tags

   Factor Xa site-3xFlag-Myc-6xHis

   Expression

   Bacterial

   Mutation

   silent mutation C to T at position 10546 (elimina…

   Promoter

   T7

   Available Since

   Nov. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pET28-His6-SUMO-cSrc-SH2-AviTag

   Plasmid

   #214230

   Purpose

   Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

   Depositor

   Neel Shah

   Article

   Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75

   Insert

   cSrc kinase SH2 domain (SRC Human)
   

   Tags

   6xHis, AviTag, and SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-GGG-HIS-CBM-Xmod-DocIII

   Plasmid

   #91698

   Purpose

   CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   GGG-HIS-CBM-Xmod-DocIII
   

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. His6-Twin-Strep-SUMO-pET28a-EfaCas2

    Plasmid

    #112794

    Purpose

    To express enterococcus faecalis Cas2 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas2
    

    Tags

    His6-Twin-Strep-SUMO

    Expression

    Bacterial

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET StrepII TEV LIC cloning vector (2R-T)

    Plasmid

    #29717

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    StrepII-TEV

    Expression

    Bacterial

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM11-SUMO3- sfGFP-KSR1-CA3 (N-KSR)

    Plasmid

    #217767

    Purpose

    Fluorescent reporter for glucosyl-ceramide (putative, mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Protein purification (6xhis tag, tev site, sumo3 …

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    CA3 domain aa 317-400

    Promoter

    T7 promoter

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

    Plasmid

    #217766

    Purpose

    Fluorescent reporter for ceramide (for bacterial expression and purification)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Protein purification (6xhis tag, tev site, sumo3 …

    Tags

    sfGFP

    Expression

    Bacterial

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    T7 promoter

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid

    #116955

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPV00554 pET21GG2-His(6)-BECN(248-450)

    Plasmid

    #136685

    Purpose

    Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

    Depositor

    Colin Garvie

    Article

    Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

    Insert

    Beclin-1 ECD/BARA (BECN1 Human)
    

    Tags

    Hexa-Histadine

    Expression

    Bacterial

    Mutation

    DNA optimized for E. coli expression

    Promoter

    T7

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-(pos36)GFP-9xGGS-Cre-6xHis

    Plasmid

    #62938

    Purpose

    Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    (ps36)GFP-9xGGS-Cre
    

    Tags

    (pos36)GFP and 6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-DUET-GST-eIF4G1-His6, eIF4E

    Plasmid

    #37232

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Inserts

    TIF4631 (TIF4631 Budding Yeast)
    CDC33 (CDC33 Budding Yeast)
    

    Tags

    GST and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pET mRuby2 LIC cloning vector (H6-mRuby2)

    Plasmid

    #86934

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mR…

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET mRuby2 LIC cloning vector (u-mRuby2)

    Plasmid

    #86932

    Purpose

    pET LIC cloning vector for yORF-mRuby2

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    mRuby2

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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