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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,193 results for: golden gate

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Showing: 4221 - 4240 of 5193 results

  1. pKS1139

    Plasmid
    #239500
    Purpose
    Express GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with high affinity SH3 peptide
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239500-plasmid-map-sequence-id-475873

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  2. pKS1024

    Plasmid
    #239495
    Purpose
    Express peroxisomal RsAS
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS-Venus-ePTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239495-plasmid-map-sequence-id-475870

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  3. pVL1176

    Plasmid
    #239537
    Purpose
    Express Venus as ER marker
    Depositor
    John Dueber
    Insert
    Ost1ss-Venus- HDEL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239537-plasmid-map-sequence-id-475869

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  4. pKS1135

    Plasmid
    #239497
    Purpose
    Express synthetic sfCherry3C small fragment membrane protein cargo and peroxisomal mTagBFP2
    Depositor
    John Dueber
    Insert
    Ost1ss-sfCherry3C(11)-Pex3_TMD(+/-6AA)-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239497-plasmid-map-sequence-id-475868

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  5. pPSG803

    Plasmid
    #239533
    Purpose
    CRISPR plasmid with Cas9 and sgRNA targeting YMR206W locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YMR206W
    Use
    CRISPR
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239533-plasmid-map-sequence-id-475867

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  6. pKS1169

    Plasmid
    #239503
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and inactive Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239503-plasmid-map-sequence-id-475865

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  7. pVL1280

    Plasmid
    #239539
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239539-plasmid-map-sequence-id-475864

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  8. pKS1025

    Plasmid
    #239496
    Purpose
    Express inactive peroxisomal RsAS as negative control
    Depositor
    John Dueber
    Insert
    Ubi-Y-RsAS(H360E)-Venus-ePTS1
    Expression
    Yeast
    Mutation
    H360E
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239496-plasmid-map-sequence-id-475861

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  9. pVL1279

    Plasmid
    #239538
    Purpose
    Express CDT1 membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239538-plasmid-map-sequence-id-475862

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  10. pVL1281

    Plasmid
    #239540
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239540-plasmid-map-sequence-id-475863

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  12. pHW1176

    Plasmid
    #229871
    Purpose
    gene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCE
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    GFP-C1-tbb-2 3'UTR::SEC
    Tags
    GFP-C
    Expression
    Worm
    Available Since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    229871-plasmid-map-sequence-id-475644

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Showing: 4221 - 4240 of 5193 results