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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,700 results for: golden gate

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Showing: 4221 - 4240 of 4700 results

  9. pNHT7

    Plasmid
    #186753
    Purpose
    A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.
    Depositor
    Bo Wang
    Article
    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002
    Type
    Empty backbone
    Use
    Synthetic Biology; Cloning backbone
    Promoter
    T7
    Available Since
    Oct. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    186753-plasmid-map-sequence-id-375095

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  18. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

    Plasmid Kit
    #1000000221
    Purpose
    End-joining ligation fidelity and bias data predicts high accuracy junction sets for Golden Gate assembly, providing a positive control for complex assembly via blue/white screening in E. coli.
    Depositor
    Eric Cantor
    Application
    Cloning and Synthetic Biology
    Vector Type
    Bacterial Expression
    Cloning Type
    Golden Gate
    Available Since
    June 1, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. PX458 V3

    Plasmid
    #226957
    Purpose
    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226957-plasmid-map-sequence-id-450777

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  20. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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Showing: 4221 - 4240 of 4700 results