We narrowed to 26,837 results for: pET

1. pET21-pelB-LaG-37

   Plasmid

   #172756

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-37; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-37
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET11b-MIF Y99F

   Plasmid

   #149439

   Purpose

   Expression of MIF Y99F in E. coli for protein production

   Depositor

   Elias Lolis

   Insert

   MIF (MIF Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y99F

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET29b-Ava3858-3856

   Plasmid

   #45745

   Depositor

   Christopher T Walsh, Emily Balskus

   Article

   Balskus et al Science. 2010 Sep 24;329(5999):1653-6. d

   Insert

   Ava3858-3856
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET42a-lambdaN+-L+-GSH

   Plasmid

   #98894

   Purpose

   Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

   Depositor

   Pascale Legault

   Article

   Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

   Insert

   lambdaN+-L+
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-pro-sumo2

   Plasmid

   #25102

   Depositor

   Guy Salvesen

   Article

   Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

   Insert

   pro-SUMO2 (SUMO2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 24, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pET30 SUMO-1 G97

   Plasmid

   #13381

   Depositor

   Mary Dasso

   Article

   Saitoh et al Curr Biol. 1998 Jan 15. 8(2):121-4.

   Insert

   SUMO-1, G97 (SUMO1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   G97 mutant: lacks 4 amino acids from the C-termin…

   Promoter

   Available since

   Nov. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

8. p3521 pET ubcH6

   Plasmid

   #8653

   Depositor

   Peter Howley

   Article

   Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

   Insert

   UbcH6 (UBE2E1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28-GFP-mHP1alpha

   Plasmid

   #181912

   Purpose

   Bacterial protein expression vector for GFP-tagged HP1alpha

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   mHP1alpha (Cbx5 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET26-Ufd1-His

    Plasmid

    #21266

    Depositor

    Hemmo Meyer

    Article

    Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

    Insert

    Ufd1 (Ufd1l Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET23-CBD-(-PP)-EGFP

    Plasmid

    #12597

    Depositor

    Klaus Hahn

    Article

    Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

    Insert

    Cdc42 Biosensor (WAS Human)
    

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    F271C

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15b-sfGO-Matryoshka-LE-LP

    Plasmid

    #100018

    Purpose

    Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    cpsfGFP-LSSmOrange
    

    Use

    Tags

    6x HIS tag

    Expression

    Bacterial

    Mutation

    Leu and Glu N-term linker and Leu and Pro C-term …

    Promoter

    T7

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-Ypet-PBD

    Plasmid

    #24533

    Depositor

    Klaus Hahn

    Article

    Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

    Insert

    PAK binding domain (PAK1 Human)
    

    Use

    Retroviral

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

14. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Use

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. RanBP2-RB3-4-pET23a

    Plasmid

    #85769

    Purpose

    bacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tag

    Depositor

    Frauke Melchior

    Article

    Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

    Insert

    RanBP2 fragment (RANBP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    fragment (amino acids 2304-3062)

    Promoter

    T7

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1-L713F

    Plasmid

    #174794

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-L713F (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM33_TNKS_TANK-peptide-bind

    Plasmid

    #178489

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    TNKS_TANK-peptide-bind (TNKS Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-pro-siRNA-V2

    Plasmid

    #111086

    Purpose

    For production of recombinant siRNAs (pro-siRNAs) in E. coli.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-ymYPET-CaURA3

    Plasmid

    #118460

    Purpose

    Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    YPET A206K, F208S, E232L, N235D

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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