We narrowed to 5,193 results for: golden gate
-
Plasmid#223172PurposeExpresses VC83_00191-yeGFP under control of CTR1 promoter and terminatorDepositorInsertCopper Transporter
TagsStreptagII and yeGFPExpressionYeastPromotersaccharomyces cerevisiae CTR1Available SinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228652PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assaysDepositorInsertDengue 4 portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228651PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assaysDepositorInsertDengue 3 portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker
Plasmid#228648PurposeBacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assayDepositorInsertDengue 1 portion of NS2B protease fused to NS3
TagsHis-Twin Strep-TevExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23
Plasmid#228645PurposeBacterial expression of the E11 3C proteaseDepositorInsertEcho virus 11 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012
Plasmid#228640PurposeBacterial expression of the Coxsackievirus 43 isoform B 3C proteaseDepositorInsertCoxsackievirus 43 isoform B 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 18, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease
Plasmid#228638PurposeBacterial expression of the Coxsackievirus 63 3C proteaseDepositorInsertCoxsackievirus 63 3C protease
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 3C protease with non-cleavable C-terminal His AviTag
Plasmid#228635PurposeBacterial expression of the A71 3C protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus A71 3C protease isoform A
TagsHis-AviExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Enterovirus A71 (EV-A71) isolate 4643-TW98 2A protease with non-cleavable C-terminal His AviTag
Plasmid#228634PurposeBacterial expression of the A71 2A protease for assays that require immobilization using biotin-streptavidin interactionDepositorInsertEnterovirus Coxsackievirus A71 2A protease
TagsHis-AviExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human Coxsackievirus A16 strain G10 2A protease
Plasmid#228632PurposeBacterial expression of the Coxsackievirus A16 (G-10) 2A protease this construct can be used for biochemical assay and to obtain crystals in the space group C1 2 1(PDB 8POA)DepositorInsertEnterovirus Coxsackievirus A16 2A protease (HEVAgp1 Coxsackievirus 16)
TagsHis-SUMOExpressionBacterialAvailable SinceFeb. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGLU_10
Plasmid#225818PurposeTn libraries Himar variant TET backboneDepositorTypeEmpty backboneUseSynthetic BiologyMutationN/AAvailable SinceFeb. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1387
Plasmid#229880PurposeNegative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTRDepositorInsertPmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
TagsGFP-C1ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1643
Plasmid#229876Purposegene trap RMCE plasmid with Tet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-On[rTetR-linker-QFAD]-act-4 3' UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1223
Plasmid#229875Purposegene trap RMCE plasmid with LexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertLexA[LexA(DBD)-linker-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1222
Plasmid#229874Purposegene trap RMCE plasmid with QF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertQF2[QF(DBD)-linker-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1221
Plasmid#229873Purposegene trap RMCE plasmid with QF-act-4 3' UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertQF-act-4 3' UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1177
Plasmid#229872Purposegene trap RMCE plasmid with Tet-Off[TetR-linker-QFAD]-act-4 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertTet-Off[TetR-QFAD]-act-4 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1134
Plasmid#229870Purposegene trap RMCE plasmid with cGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertcGAL[cGAL(DBD)-linker-QFAD]-tbb-2 3'UTR::SEC
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW622
Plasmid#229868PurposeEntry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)DepositorInsertreverse FRT3 (rFRT3)
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
dSpyCas9-E566-GS-LOV2-VPR
Plasmid#231576PurposePotent light-deactivated CRISPRaDepositorInsertdSpyCas9-VPR with an AsLOV2 insertion behind E566
UseCRISPRExpressionMammalianAvailable SinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only