We narrowed to 597 results for: pFAST

1. pFB-GTE-COI1

   Plasmid

   #29516

   Depositor

   Ning Zheng

   Article

   Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

   Insert

   COI1 (COI1 Mustard Weed)
   

   Use

   Tags

   GST and TEV cleavage site

   Expression

   Insect

   Mutation

   Note that the first AA in TEV site is R, not E

   Promoter

   Available since

   May 26, 2011

   Availability

   Academic Institutions and Nonprofits only

2. CD45 sp-SNAPf-CD45R0Ex-His10 (XSB316)

   Plasmid

   #78509

   Purpose

   Construct for expressing CD45R0 (extracellular domain) His10 in Sf9

   Depositor

   Ron Vale

   Article

   Su et al Science. 2016 Apr 29;352(6285):595-9. do

   Insert

   CD45R0Ex (PTPRC Human)
   

   Use

   Tags

   His10 and SNAPf

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ClCry4 Y319D

   Plasmid

   #160649

   Purpose

   Expression of protein for various experiments including finding interaction partners

   Depositor

   Joseph Takahashi

   Article

   Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

   Insert

   Clcry4 PHR Y319D
   

   Use

   Tags

   Hexa his

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFBD-Akt1(WT)

   Plasmid

   #86561

   Purpose

   Expresses human full-length Akt1(WT)

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-CmTMC1truncated

   Plasmid

   #154908

   Purpose

   For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-CmTMC1truncated
   

   Use

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-243 and 843-969

   Promoter

   Available since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-MuTMC2truncated

   Plasmid

   #154909

   Purpose

   For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-MuTMC2truncated
   

   Use

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-179 and 691-716

   Promoter

   Available since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. IMPT-11371

   Plasmid

   #236191

   Purpose

   Express GPR119 in insect cells

   Depositor

   Vadim Cherezov

   Article

   Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

   Insert

   GPR119 (GPR119 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 3xMS-TR-100-PURO HRD

   Plasmid

   #207582

   Purpose

   homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
   

   Use

   Tags

   3xMS2

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RcSxph E540A

   Plasmid

   #194019

   Purpose

   Protein expression and characterization

   Depositor

   Dan Minor

   Article

   Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

   Insert

   Rana catesbeiana Saxiphilin
   

   Use

   Tags

   His10 and mEGFP,

   Expression

   Insect

   Mutation

   E540A

   Promoter

   Polyhedrin

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RcSxph E540D

    Plasmid

    #194020

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540D

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RcSxph E540Q

    Plasmid

    #194021

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    E540Q

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. RcSxph Y558A

    Plasmid

    #194022

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RcSxph Y558F

    Plasmid

    #194023

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558F

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RcSxph Y558I

    Plasmid

    #194024

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    Y558I

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RcSxph F561A

    Plasmid

    #194025

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F561A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RcSxph T563A

    Plasmid

    #194026

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    T563A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RcSxph P727A

    Plasmid

    #194027

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    P727A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RcSxph I782A

    Plasmid

    #194028

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    I782A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RcSxph I782F

    Plasmid

    #194029

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    I782F

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RcSxph F784A

    Plasmid

    #194030

    Purpose

    Protein expression and characterization

    Depositor

    Dan Minor

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

    Insert

    Rana catesbeiana Saxiphilin
    

    Use

    Tags

    His10 and mEGFP,

    Expression

    Insect

    Mutation

    F784A

    Promoter

    Polyhedrin

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge