We narrowed to 16,262 results for: grn

1. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

   Plasmid

   #67977

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-pegRNA-puro backbone

   Plasmid

   #214085

   Purpose

   Lentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6, EF1a

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV sgRNA Expression Plasmid

   Plasmid

   #174540

   Purpose

   Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

   Depositor

   Ormond MacDougald

   Article

   Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

   Type

   Empty backbone

   Use

   AAV

   Tags

   None

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. v1em-Cterm-PE2max-U6-pegRNA

   Plasmid

   #198733

   Purpose

   AAV genome encoding C-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   NpuC-CtermPE2max
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. v1em-Nterm-PE2max-U6-sgRNA

   Plasmid

   #198732

   Purpose

   AAV genome encoding N-terminal PE2max and U6 expression cassette

   Depositor

   David Liu

   Article

   Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

   Insert

   Nterm PE2max-NpuN
   

   Use

   AAV

   Promoter

   EFS

   Available Since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBT-PE2-PuroTK-pegRNA_GG

   Plasmid

   #173222

   Purpose

   piggyBac transposon containing both PE2 and a pegRNA cloning site.

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   PE2
   PuroTK
   pegRNA cloning cassette
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CMV, P2A, and hU6

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti U6-sgRNA/EF1a-mCherry

   Plasmid

   #114199

   Purpose

   Lentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   sgRNA scaffold
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

    Plasmid

    #217635

    Purpose

    AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

    Depositor

    Martin Kampmann

    Article

    Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

    Insert

    mU6-sgRNA, Handle sequence, EF1a-mTagBFP2
    

    Use

    AAV

    Promoter

    EF1alpha and mU6

    Available Since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. p426-SNR52p-gRNA.CAN1.Y-SUP4t

    Plasmid

    #43803

    Purpose

    Encodes a gRNA that targets Can1.Y in yeast.

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    CAN1.y gRNA (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    March 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

    Plasmid

    #218527

    Purpose

    sgRNA targeting human EIF2AK2/PKR

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK2 (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCRISPR v2-Nr4a1 double gRNAs

    Plasmid

    #162791

    Purpose

    Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Promoter

    hU6

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

    Plasmid

    #218666

    Purpose

    sgRNA targeting human EIF2AK3/PERK

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK3 (EIF2AK3 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-U6-sgRNA-BFP-Puro

    Plasmid

    #229014

    Purpose

    Perturb Seq lentiviral sgRNA vector

    Depositor

    Gary Hon

    Article

    Sivakumar et al bioRxiv [Preprint]. 2025 Jan 23:2025.01.

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

    Plasmid

    #218529

    Purpose

    sgRNA targeting human EIF2AK1/HRI

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    EIF2AK1 (EIF2AK1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY0663 ACTB atgRNA with v2 scaffold

    Plasmid

    #179108

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA with v2 scaffold

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA with v2 scaffold
    

    Use

    CRISPR

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPUR-mU6-sgRNA-2XPP7

    Plasmid

    #194504

    Purpose

    Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

    Depositor

    Chunqing Song

    Article

    Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mU6-sgRNA-CLTA-mCherry

    Plasmid

    #240637

    Purpose

    Vector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.

    Depositor

    James Nuñez

    Article

    Xu et al Nat Commun. 2025 Aug 26;16(1):7948. doi:

    Insert

    puro-T2A-mCherry
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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