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Showing: 1 - 20 of 13718 results

  1. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  10. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  17. px552-U6-gRNA1-U6-gRNA2-CMV-eGFP

    Plasmid
    #211760
    Purpose
    Paired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.
    Depositor
    Glenn Yiu
    Insert
    gRNA1 and gRNA2 targeting VEGF-A (VEGFA M. mulatta (rhesus macaque), Mouse, Human)
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    211760-plasmid-map-sequence-id-421239

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Showing: 1 - 20 of 13718 results