We narrowed to 16,198 results for: grna

1. pX459-HypaCas9-mR2-AAVS1_sgRNA

   Plasmid

   #183890

   Purpose

   pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

   Depositor

   Alisa Piekny

   Article

   Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

   Insert

   AAVS1 sgRNA spacer (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV2-U6gRNA5(hADAR1_2g)-PGKpuro2ABFP-W

   Plasmid

   #208434

   Purpose

   Lentiviral gRNA plasmid targeting human ADAR1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Yee et al Mol Carcinog. 2025 Jun;64(6):1066-1077.

   Insert

   ADAR1 (ADAR Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   human U8

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. 7b sgRNA for EJ7-GFP reporter

   Plasmid

   #113624

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7b sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

   Plasmid

   #112925

   Purpose

   Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   hU6 promoter, gRNA-SAM expression cassette
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-U6-dgRNA-CAG-MPH

   Plasmid

   #106259

   Purpose

   Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   MS2-P65-HSF1 (HSF1 Synthetic, Human)
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC57-Simple-gRNA backbone

   Plasmid

   #51306

   Purpose

   gRNA expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Type

   Empty backbone

   Use

   CRISPR; Xenopus expression

   Promoter

   T7

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmAG-W

   Plasmid

   #67976

   Purpose

   CRISPR gRNA expression vector with an improved scaffold and puro/mAG markers

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKpuro2AmAG cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Promoter

   human U6 promoter, mouse PGK promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG79-attL1-pegRNA-attL2

   Plasmid

   #213050

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVi U6_gRNA CMV_SadCas9-KRAB

   Plasmid

   #214609

   Purpose

   All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

   Depositor

   Ralf Gilsbach

   Article

   Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

   Inserts

   gRNA
   SadCas9
   

   Use

   AAV

   Tags

   NP NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   CMV and U6

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP

    Plasmid

    #67991

    Purpose

    CRISPR gRNA expression vector with an improved l scaffold and puro/BFP markers without WPRE

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette with an improved scaffold, PGKpuro2ABFP cassette
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLQ14107 fwYellow-targeting sgRNA

    Plasmid

    #239456

    Purpose

    guide for fwYellow

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting fwYellow
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ14112 tyrosinase-targeting sgRNA

    Plasmid

    #239751

    Purpose

    guide for tyrosinase

    Depositor

    Stanley Qi

    Article

    Collins et al Nat Commun. 2024 Aug 3;15(1):6563. doi:

    Insert

    guide targeting tyrosinase
    

    Use

    CRISPR; Cell-free system using bacterial extract

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. HH-gRNA-HDV-Shuttle-Vector

    Plasmid

    #169098

    Purpose

    Backbone plasmid to generate HH-gRNA-HDV segment

    Depositor

    Marianne Bronner

    Article

    Gandhi et al Development. 2021 Mar 9. pii: dev.193565

    Insert

    HH-gRNAbackbone-HDV
    

    Expression

    Mammalian

    Promoter

    NA

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Puro G3BP1-sgRNA

    Plasmid

    #196197

    Purpose

    Cas9 + gRNA expression to edit G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 (G3BP1 Human)
    

    Use

    CRISPR

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-sp-sgRNA-RNF2_+41nick

    Plasmid

    #135958

    Purpose

    S. pyogenes sgRNA for +41 nick in RNF2

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_+41 nicking sgRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MiniCoopR 2xU6:gRNA, mitfa:Cas9

    Plasmid

    #118844

    Purpose

    Targets 2 genes and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Type

    Empty backbone

    Use

    CRISPR; Tol2

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W

    Plasmid

    #67985

    Purpose

    Cas9 activity reporter (control) with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF1-alpha

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330GFP-hU6-gRNA-hSpCas9

    Plasmid

    #128385

    Purpose

    GFP positive gRNA/hSpCas9 construct

    Depositor

    Budd A. Tucker

    Article

    Giacalone et al Curr Protoc Stem Cell Biol. 2018 Feb 28;

    Insert

    GFP, gRNA and hSpCas9
    

    Use

    CRISPR

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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