We narrowed to 16,198 results for: grna

1. pUb-Cas9-EGFP_cU6:6-sgRNA

   Plasmid

   #190597

   Purpose

   The plasmid encodes S. pyogenes Cas9 and eGFP separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter.

   Depositor

   Claudia Rueckert

   Article

   Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   CRISPR

   Tags

   EGFP - separated by T2A

   Promoter

   Aedes aegypti polyubiquitin and Culex quinquefasc…

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWS3799-gRNA-Csy4-template

   Plasmid

   #182827

   Purpose

   SpCas9 gRNA-Csy4 template

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Insert

   SpCas9 gRNA scaffold + Csy4 sequence
   

   Use

   CRISPR

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM254 - sgRNA mosTI neoR

   Plasmid

   #159831

   Purpose

   Single copy or array insertion by MosTI using split NeoR selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI neoR
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLentiCrisprV2 sgRNA hCRY1 c-term

   Plasmid

   #179453

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hCRY1 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human CRY1
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBx-Spas-sgRNA-Kan

   Plasmid

   #105233

   Purpose

   Plasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescens

   Depositor

   Kristala Prather

   Article

   Tan et al J Bacteriol. 2018 Mar 12;200(7). pii: JB

   Insert

   S. pasteurianus sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(SMARCA2(47))-PGKpuro2ABFP-W

   Plasmid

   #200510

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA2(47) (SMARCA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNTI661 pRPR1(TetO)-sgRNA

   Plasmid

   #139475

   Purpose

   Vector for tet-regulated sgRNA expression in yeast

   Depositor

   Nicholas Ingolia

   Article

   McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

   Insert

   single guide RNA scaffold
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pRPR1(TetO)

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR-U6-gRNA-miniCMV-TdTomato

   Plasmid

   #192656

   Purpose

   A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Expression

   Mammalian

   Promoter

   U6, miniCMV

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid

    #55200

    Purpose

    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0043-PspCas13b-gRNA LINE1

    Plasmid

    #223699

    Purpose

    gRNA of dPspCas13b-FTO targeting LINE1

    Depositor

    Chuan He

    Article

    Wei et al Science. 2022 May 27;376(6596):968-973.

    Insert

    gRNA target sequence for LINE1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-puro U6 sgRNA CAG

    Plasmid

    #50927

    Purpose

    U6 driven sgRNA negative control

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    negative control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMD19-PBBa_J23117-sgRNA-Spr

    Plasmid

    #190793

    Purpose

    Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

    Depositor

    Paul Hudson

    Article

    Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

    Insert

    sgRNA (dummy)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BBa_J23117

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W

    Plasmid

    #67975

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/ZsG markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AZsG cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. px335-sgRNA-EF(Cas9n)-Dnmt3b-R

    Plasmid

    #122333

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. px335-sgRNA-EF(Cas9n)-Dnmt3b-L

    Plasmid

    #122334

    Purpose

    Expresses sgRNA targeting mouse Dnmt3b and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Dnmt3b
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDY0207 ACTB atgRNA with v1 scaffold

    Plasmid

    #179107

    Purpose

    ACTB N-term PBS 13 RT 29 Bxb1 AttB 46 atgRNA

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

    Insert

    ACTB N-term PBS 13 RT 29 AttB 46 atgRNA
    

    Use

    CRISPR

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-sp-sgRNA-mDNMT1_nick-lenti

    Plasmid

    #135959

    Purpose

    S. pyogenes nicking sgRNA for mouse DNMT1

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    mDNMT1_nick
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUb-Cas9-mCherry_cU6:6-sgRNA

    Plasmid

    #190598

    Purpose

    The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

    Depositor

    Claudia Rueckert

    Article

    Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

    Inserts

    Cas9
    sgRNA
    

    Use

    CRISPR

    Tags

    mCherry - separated by T2A

    Promoter

    Aedes aegypti polyubiquitin and Culex quinquefasc…

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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