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We narrowed to 9,957 results for: pET

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Showing: 4761 - 4780 of 9957 results

  1. pETcon-pGAL1-Aga2-Amyloid β42-c_myc

    Plasmid
    #225223
    Purpose
    Yeast optimized human Amyloid β42 fused with aga2 protein gene under gal1 promoter for the expression of Amyloid β42 in yeast surface display.
    Depositor
    Urartu Seker
    Publication
    Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360
    Inserts
    Amyloid β42 (APP Human)
    Aga2 (AGA2 Budding Yeast)
    Tags
    HA Tag and c-myc Tag
    Expression
    Yeast
    Promoter
    GAL1
    Available since
    Feb. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    225223-plasmid-map-sequence-id-460494

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  2. pET28-His6-SUMO-cSrc-SH2-AviTag

    Plasmid
    #214230
    Purpose
    Bacterial expression of SH2 domain of the cSrc kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections
    Depositor
    Neel Shah
    Publication
    Li et al Elife. 2023 Mar 16;12:e82345. doi: 10.75
    Insert
    cSrc kinase SH2 domain (SRC Human)
    Tags
    6xHis, AviTag, and SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    214230-plasmid-map-sequence-id-429073

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  8. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid
    #153446
    Purpose
    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
    Depositor
    Michael Nash
    Publication
    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:
    Insert
    Rc.CohE
    Tags
    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available since
    July 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    153446-plasmid-map-sequence-id-306487

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  10. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116956
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Publication
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    116956-plasmid-map-sequence-id-227069

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  11. pET11a asyn Q79N (Q109H) (TAC-->TAT)

    Plasmid
    #105736
    Purpose
    Bacterial expression of mutant human alpha synuclein
    Depositor
    Hilal Lashuel
    Insert
    alpha-synuclein Q79N (Q109H) (TAC-->TAT) (SNCA Human)
    Expression
    Bacterial
    Mutation
    Q79N/Q109H
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    105736-plasmid-map-sequence-id-207269

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  13. pET21-10XHis-GST-HRV-Her1-dL5

    Plasmid
    #73216
    Purpose
    Bacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    10XHis-GST-HRV-Her1-dL5 (EGFR Schistosoma japonicum, Human, Synthetic)
    Tags
    10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73216-plasmid-map-sequence-id-137065

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  14. pET21-10XHis-GST-HRV-dL5-Her1

    Plasmid
    #73215
    Purpose
    Bacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Publication
    Wang et al Bioconjug Chem. 2015 Jan 21;26(1):137-44
    Insert
    EGFR (EGFR Schistosoma japonicum, Human, Synthetic)
    Tags
    10XHis-GST-HRV and The dL5 FAP and Her1 affibody …
    Expression
    Bacterial
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    T7
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73215-plasmid-map-sequence-id-137064

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  15. pETDuet1_BCL2L13(1-465aa)-EGFP-TEV-GST (I224A/L227A/W276A/I279A/I307A/V310A; deltaLIR1+3+4)

    Plasmid
    #223784
    Purpose
    Expression of recombinant protein for purification
    Depositor
    Sascha Martens
    Publication
    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038
    Insert
    BCL2L13 soluble part (BCL2L13 Human)
    Tags
    EGFP-TEV-GST
    Expression
    Bacterial
    Mutation
    I224A/L227A/W276A/I279A/I307A/V310A; deletion of …
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    223784-plasmid-map-sequence-id-448415

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  17. pCJ200

    Plasmid
    #162673
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S136C and a sequence Y75-F104 to introduce a 6th…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162673-plasmid-map-sequence-id-321521

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  18. pCJ220

    Plasmid
    #162686
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224W, C529S; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162686-plasmid-map-sequence-id-321468

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  19. pCJ221

    Plasmid
    #162687
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.
    Depositor
    Gregg Beckham
    Publication
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaG251-T472, C224H, C529F; codon optimized for…
    Promoter
    T7/lac
    Available since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    162687-plasmid-map-sequence-id-321457

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  20. pET28-MHL_IFIH1:306-1017

    Plasmid
    #247536
    Purpose
    E. coli expression for protein production. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    IFIH1 (IFIH1 Human)
    Tags
    MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    VAR_021594 (H843R) and VAR_021595 (A946T) from te…
    Promoter
    T7
    Available since
    Sept. 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    247536-plasmid-map-sequence-id-515932

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Showing: 4761 - 4780 of 9957 results