We narrowed to 5,346 results for: golden gate

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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-Bxb1-I87L, A369P, E434G

Plasmid

#232750

Purpose

IVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)

Depositor

Kate Galloway

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

Bxb1

Use

In vitro transcription

Tags

HA and SV40 NLS

Mutation

I87L, A369P, E434G

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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dSpyCas9-E566-GS-LOV2-VPR

Plasmid

#231576

Purpose

Potent light-deactivated CRISPRa

Depositor

Dominik Niopek

Insert

dSpyCas9-VPR with an AsLOV2 insertion behind E566

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pM-mKate2

Plasmid

#228629

Purpose

Mammalian expression of mKate2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mKate2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pM-LSSmOrange

Plasmid

#228619

Purpose

Mammalian expression of LSSmOrange and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

LSSmOrange

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3328 PB-sfGFP-WPRE-target

Plasmid

#226676

Purpose

PiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

sfGFP-WPRE-site3target

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only
pM-mBeRFP

Plasmid

#228620

Purpose

Mammalian expression of mBeRFP and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mBeRFP

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mTFP1

Plasmid

#228621

Purpose

Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mTFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-CyOFP1

Plasmid

#228623

Purpose

Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

CyOFP1

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mOrange2

Plasmid

#228627

Purpose

Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mOrange2

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pM-mRuby3

Plasmid

#228628

Purpose

Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mRuby3

Expression

Mammalian

Mutation

We introduced some silent mutations to avoid esse…

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crCoV-Mutiplex_EF1a-BFP

Plasmid

#224788

Purpose

SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Article

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crCoV20, crCoV21, crCoV24

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available Since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3401 BtoApegRNA-sfGFP

Plasmid

#226667

Purpose

PiggyBac cargo vector encoding B to A pegRNA marked with sfGFP

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

BtoA pegRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG-sgRNA.PRDM1-ZEB2_CRISPRd

Plasmid

#216171

Purpose

Express the gRNA targeting the PRDM1-binding site in the human ZEB2 locus for the CRISPRd experiment

Depositor

Makiko Iwafuchi

Article

Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

Insert

sgRNA- PRDM1.bs- ZEB2.locus (PRDM1 Human)

Use

Lentiviral

Tags

EGFP

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm1

Plasmid

#222911

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 1 (NPM2 Synthetic, Human)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm4

Plasmid

#222914

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 4 (NPM2 Synthetic, Human)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm3

Plasmid

#222913

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 3 (NPM2 Synthetic, Human)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_NPM2_cterm2

Plasmid

#222912

Purpose

Cas9/sgRNA plasmid for targeting NPM2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NPM2 sgRNA 2 (NPM2 Synthetic, Human)

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Gln-tRNA-gRNA scaffold for library 1&2

Plasmid

#192367

Purpose

tRNA sequence provider (for checkpoint and immune library)

Depositor

Lijia Ma

Article

Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

Insert

Gln tRNA-gRNA scaffold

Use

For golden gate assembly

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7

Plasmid

#186753

Purpose

A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Type

Empty backbone

Use

Synthetic Biology; Cloning backbone

Promoter

T7

Available Since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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