Facile, high-throughput protein purification enabled by high-efficiency protease elution.
Cárcamo Noriega EN, Truebridge IS, Teets FD 3rd, Bowman JW 3rd, Rodriguez N, Howard TA, Bahl CD
Protein Sci. 2026 Sep;35(9):e70746. doi: 10.1002/pro.70746.
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PubMed
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Article
These materials are included in the Protein Purification Optimization Kit Using SUMO Protease Elution.
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 233685 | pAIP1 | Engineered SUMO protease expression plasmid designed for efficient protease-mediated elution. |
| 233686 | pAIP4 | Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion. |
| 234143 | pAIP4_2D5J | Heterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coli |
| 234144 | pAIP4_1EDG | Heterologous protein expression of GUNA_RUMCH in Escherichia coli |
| 234145 | pAIP4_2GAS | Heterologous protein expression of isoflavone reductase (IFR) in Escherichia coli |
| 234146 | pAIP4_1H6T | Heterologous protein expression of internalin B (InlB) in Escherichia coli |
| 234147 | pAIP4_2H2Z | Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli |
| 234148 | pAIP4_5IHW | Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli |
| 234149 | pAIP4_2OKT | Heterologous protein expression of o-succinylbenzoate synthase (OSB synthase) in Escherichia coli |
| 234150 | pAIP4_5YPV | Heterologous protein expression of malonyl CoA-acyl carrier protein transacylase in Escherichia coli |
| 234151 | pAIP4_1XSZ | Heterologous protein expression of RalF in Escherichia coli |
| 234152 | pAIP4_6BIO | Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli |
| 234153 | pAIP4_4WDC | Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli |
| 234154 | pAIP4_5EF9 | Heterologous protein expression of polymerase basic protein 2 (RNA-directed RNA polymerase subunit P3) in Escherichia coli |
| 234155 | pAIP4_5MC7 | Heterologous protein expression of coatomer subunit zeta-1 (Zeta-1-coat protein) (Zeta-1 COP) in Escherichia coli |
| 234156 | pAIP4_3RZY | Heterologous protein expression of fatty acid-binding protein, adipocyte (ALBP) in Escherichia coli |
| 234157 | pAIP4_3K1H | Heterologous protein expression of O25709_HELPY in Escherichia coli |
| 234158 | pAIP4_4ZHW | Heterologous protein expression of YfiB in Escherichia coli |
| 234159 | pAIP4_1EYH | Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli |
| 234160 | pAIP4_2H3L | Heterologous protein expression of erbin (Densin-180-like protein) (Erbb2-interacting protein) in Escherichia coli |
| 234161 | pAIP4_4NFB | Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli |
| 234162 | pAIP4_1AKO | Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli |
| 234163 | pAIP4_4CFI | Heterologous protein expression of flagellin in Escherichia coli |
| 234164 | pAIP4_4RZ9 | Heterologous protein expression of pre-mRNA-splicing factor 38A in Escherichia coli |
| 234165 | pAIP4_3V46 | Heterologous protein expression of cell division control protein 73 in Escherichia coli |
| 234166 | pAIP4_1UXZ | Heterologous protein expression of cellulase B in Escherichia coli |
| 234167 | pAIP4_1TJ6 | Heterologous protein expression of sprouty-related, EVH1 domain-containing protein 1 (Spred-1) in Escherichia coli |
| 234168 | pAIP4_1BKR | Heterologous protein expression of spectrin beta chain, non-erythrocytic 1 (Beta-II spectrin) in Escherichia coli |
| 234169 | pAIP4_1HKA | Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli |
| 234170 | pAIP4_2JIK | Heterologous protein expression of synaptojanin-2-binding protein in Escherichia coli |
| 234171 | pAIP4_5GUQ | Heterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coli |
| 234172 | pAIP4_1AKZ | Heterologous protein expression of uracil-DNA glycosylase (UDG) in Escherichia coli |
| 234173 | pAIP4_2EYI | Heterologous protein expression of alpha-actinin-1 in Escherichia coli |
| 234174 | pAIP4_4QMD | Heterologous protein expression of envoplakin in Escherichia coli |
| 234175 | pAIP4_4O6G | Heterologous protein expression of uncharacterized protein in Escherichia coli |
| 234176 | pAIP4_3EYE | Heterologous protein expression of N-acetylgalactosamine-specific enzyme IIB component of PTS in Escherichia coli |
| 234177 | pAIP4_4Q2S | Heterologous protein expression of C20G4.08 in Escherichia coli |
| 234178 | pAIP4_2OHW | Heterologous protein expression of YueI in Escherichia coli |
| 234179 | pAIP4_3ONH | Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli |
| 234180 | pAIP4_1EW4 | Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli |
| 234181 | pAIP4_1KQX | Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli |
| 234182 | pAIP4_3A7L | Heterologous protein expression of glycine cleavage system H protein in Escherichia coli |
| 234183 | pAIP4_5KUW | Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli |
| 234184 | pAIP4_5H28 | Heterologous protein expression of oxysterol-binding protein homolog 1 in Escherichia coli |
| 234185 | pAIP4_3H6Q | Heterologous protein expression of macrocypin-1a in Escherichia coli |
| 234186 | pAIP4_5MDT | Heterologous protein expression of rpb7-binding protein seb1 in Escherichia coli |
| 234187 | pAIP4_4QB4 | Heterologous protein expression of thermonuclease (TNase) in Escherichia coli |
| 234188 | pAIP4_5EIP | Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli |
| 234189 | pAIP4_5HFD | Heterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coli |
| 234190 | pAIP4_2PPN | Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP1A (PPIase FKBP1A) in Escherichia coli |
| 234191 | pAIP4_1NG6 | Heterologous protein expression of YqeY in Escherichia coli |
| 234192 | pAIP4_3KT9 | Heterologous protein expression of aprataxin in Escherichia coli |
| 234193 | pAIP4_5M2Y | Heterologous protein expression of B7LG64_ECO55 in Escherichia coli |
| 234194 | pAIP4_4A02 | Heterologous protein expression of lytic chitin monooxygenase in Escherichia coli |
| 234195 | pAIP4_4REH | Heterologous protein expression of major latex-like protein in Escherichia coli |
| 234196 | pAIP4_2A4D | Heterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coli |
| 234197 | pAIP4_1ZHV | Heterologous protein expression of aspartate kinase in Escherichia coli |
| 234198 | pAIP4_5HFQ | Heterologous protein expression of bromodomain-containing protein 2 in Escherichia coli |
| 234199 | pAIP4_3HNX | Heterologous protein expression of cyanovirin-N homolog (CV-N homolog) in Escherichia coli |
| 234200 | pAIP4_1TP9 | Heterologous protein expression of peroxiredoxin-2 (Prx) in Escherichia coli |
| 234201 | pAIP4_3TOW | Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli |
| 234202 | pAIP4_5NJO | Heterologous protein expression of putative cupin_2 domain-containing isomerase in Escherichia coli |
| 234203 | pAIP4_1TJE | Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli |
| 234204 | pAIP4_5Y33 | Heterologous protein expression of alginate lyase in Escherichia coli |
| 234205 | pAIP4_5HIH | Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli |
| 234206 | pAIP4_1JL1 | Heterologous protein expression of ribonuclease HI (RNase HI) in Escherichia coli |
| 234207 | pAIP4_1LU4 | Heterologous protein expression of soluble secreted antigen MPT53 in Escherichia coli |
| 234208 | pAIP4_2NT3 | Heterologous protein expression of response regulator homolog in Escherichia coli |
| 234209 | pAIP4_1KAF | Heterologous protein expression of middle transcription regulatory protein motA in Escherichia coli |
| 234210 | pAIP4_2WWE | Heterologous protein expression of PI3K-C2-gamma in Escherichia coli |
| 234211 | pAIP4_1U8T | Heterologous protein expression of flagellar motor switch protein FliM in Escherichia coli |
| 234212 | pAIP4_4ACJ | Heterologous protein expression of Wufb25h12 in Escherichia coli |
| 234213 | pAIP4_5XBC | Heterologous protein expression of 3C-like proteinase in Escherichia coli |
| 234214 | pAIP4_1UKF | Heterologous protein expression of cysteine protease avirulence protein AvrPphB in Escherichia coli |
| 234215 | pAIP4_3ILS | Heterologous protein expression of norsolorinic acid synthase (NSAS) in Escherichia coli |
| 234216 | pAIP4_1FL0 | Heterologous protein expression of aminoacyl tRNA synthase complex-interacting multifunctional protein 1 in Escherichia coli |
| 234217 | pAIP4_1ELK | Heterologous protein expression of target of Myb protein 1 in Escherichia coli |
| 234218 | pAIP4_2JHX | Heterologous protein expression of rho GDP-dissociation inhibitor 1 (Rho GDI alpha) in Escherichia coli |
| 234219 | pAIP4_2RH3 | Heterologous protein expression of protein virC2 in Escherichia coli |
| 234220 | pAIP4_2H7W | Heterologous protein expression of chagasin in Escherichia coli |
| 234221 | pAIP4_3CX2 | Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli |
| 234222 | pAIP4_2AWG | Heterologous protein expression of peptidyl-prolyl cis-trans isomerase FKBP8 (PPIase FKBP8) in Escherichia coli |
| 234223 | pAIP4_4HS5 | Heterologous protein expression of iron-sulfur cluster assembly protein CyaY in Escherichia coli |
| 234224 | pAIP4_6F2Q | Heterologous protein expression of galectin-3 (Gal-3) (CBP 35) in Escherichia coli |
| 234225 | pAIP4_3KB5 | Heterologous protein expression of tripartite motif-containing protein 72 (Mg53) in Escherichia coli |
| 234226 | pAIP4_5F66 | Heterologous protein expression of peptidyl-prolyl cis-trans isomerase A (PPIase A) in Escherichia coli |
| 234227 | pAIP4_3OBS | Heterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coli |
| 234228 | pAIP4_1T3Y | Heterologous protein expression of coactosin-like protein in Escherichia coli |
| 234229 | pAIP4_4IPC | Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli |
| 234230 | pAIP4_3D1B | Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli |
| 234231 | pAIP4_3S60 | Heterologous protein expression of lectin OAA in Escherichia coli |
| 234232 | pAIP4_2ESK | Heterologous protein expression of ubiquitin-conjugating enzyme E2 D2 in Escherichia coli |
| 234233 | pAIP4_4J5Q | Heterologous protein expression of ADP-ribose glycohydrolase OARD1 in Escherichia coli |
| 234234 | pAIP4_6WEY | Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli |
| 234235 | pAIP4_1YW5 | Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli |
| 234236 | pAIP4_3W4Q | Heterologous protein expression of beta-lactamase in Escherichia coli |
| 234237 | pAIP4_6IYE | Heterologous protein expression of peptidyl-tRNA hydrolase (PTH) in Escherichia coli |
| 234238 | pAIP4_9C74 | Heterologous protein expression of variant of super folded GFP in Escherichia coli |