Skip to main content
Addgene
Open Menu

pAIP53
(Plasmid #258140)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258140 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pET29b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smt3
  • Entrez Gene
    SMT3 (a.k.a. YDR510W)
  • Promoter T7
  • Tag / Fusion Protein
    • HisTag (N terminal on insert) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAIP53 was a gift from Christopher Bahl (Addgene plasmid # 258140 ; http://n2t.net/addgene:258140 ; RRID:Addgene_258140)

  • For your References section:

    Facile, high-throughput protein purification enabled by high-efficiency protease elution. Cárcamo Noriega EN, Truebridge IS, Teets FD 3rd, Bowman JW 3rd, Rodriguez N, Howard TA, Bahl CD. Protein Sci. 2026 Sep;35(9):e70746. doi: 10.1002/pro.70746. 10.1002/pro.70746 PubMed 42555168
Related items: