We narrowed to 16,198 results for: grna

1. pU6-pegRNA-LRRK2-G2019S-3a

   Plasmid

   #180432

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6-pegRNA-SNCA-A53T

   Plasmid

   #181738

   Purpose

   Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Human U6

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiGuide-Puro/CD73-gRNA4

   Plasmid

   #132588

   Purpose

   Lentiviral gRNA vector targeting human CD73

   Depositor

   Robert Sobol

   Article

   Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.

   Insert

   CD73 (NT5E Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(GATA3(13))-PGKpuro2ABFP-W

   Plasmid

   #208548

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(13) (GATA3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-U6gRNA5(GATA3(12))-PGKpuro2ABFP-W

   Plasmid

   #208547

   Purpose

   Lentiviral vector expressing gRNA targeting human GATA3

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   GATA3(12) (GATA3 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sgRNA2-2-RPB1-C-term

   Plasmid

   #195109

   Purpose

   Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 C-term. Puromycin selection (2A-fusion).

   Depositor

   Job Dekker

   Article

   Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

   Insert

   sgRNA against last exon of RPB1 (C-terminal)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SauCas9 EMX1-nicking sgRNA

   Plasmid

   #169860

   Purpose

   SauCas9 nicking sgRNA for EMX1

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SauCas9 EMX1-nicking sgRNA
   

   Expression

   Mammalian

   Promoter

   human U6 promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTBL437 AAVS1 EQR sgRNA

   Plasmid

   #126447

   Purpose

   To cut the human AAVS1 locus for integration.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human AAVS1 safe-harbor locus with NGAG PAM
   

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-NQL005-SOX2-sgRNA

    Plasmid

    #175553

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA scaffold-spacer-human U6

    Plasmid

    #214697

    Purpose

    provide "sgRNA scaffold-spacer-human U6" fragment

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    sgRNA scaffold-spacer-human U6
    

    Use

    CRISPR

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-sgRNA4-CTCF-3p-UTR

    Plasmid

    #195106

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF 3' UTR region
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-sgRNA3-CTCF-3p-UTR

    Plasmid

    #195105

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting downstream of the human CTCF 3'UTR. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF 3' UTR region
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-sgRNA2-CTCF-prom

    Plasmid

    #195104

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF promoter
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-sgRNA1-CTCF-prom

    Plasmid

    #195103

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting upstream of the human CTCF promoter. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against CTCF promoter
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX459-HypaCas9-mR2-ANLN_sgRNA

    Plasmid

    #183876

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro U6 sgRNA Oct4A -12

    Plasmid

    #50921

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -12 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pM115: CasRx control presgRNA

    Plasmid

    #166867

    Purpose

    U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    non-targeting presgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBbdCas9S(-10TC)_Psyn-sgRNA500

    Plasmid

    #149583

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CDKN1A (p21) targeting gRNA

    Plasmid

    #215319

    Purpose

    Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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