168,037 results

1. pCRIS-PITChv2-FBL

   Plasmid

   #63672

   Purpose

   PITCh donor vector for EGFP-2A-PuroR knock-in into human FBL locus

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   EGFP-2A-PuroR
   

   Use

   CRISPR

   Promoter

   Promoterless

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEYFP-N3-EPAC1

   Plasmid

   #113110

   Purpose

   Human EPAC1 gene was fused in-frame and upstream from the enhanced YFP gene in pEYFP-N3 vector (Clonetech).

   Depositor

   Xiaodong Cheng

   Article

   Mei et al J Biol Chem. 2002 Mar 29;277(13):11497-5

   Insert

   RapGEF3 (RAPGEF3 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   PCMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 765 pME nls mCerulean

   Plasmid

   #195958

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean nuclear
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV1)

   Viral Prep

   #162380-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Promoter

   CAG

   Available Since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

5. pLV-puro-N-Flag-h-SNRNP40

   Plasmid

   #134254

   Purpose

   Lentivector encoding Flag-tagged human SNRNP40

   Depositor

   Bruce Beutler

   Article

   Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

   Insert

   SNRP40 (SNRNP40 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. VN-Tau (P301L)

   Plasmid

   #87634

   Purpose

   expresses hTau fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Insert

   MAPT (MAPT Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Mutation P301L

   Promoter

   CMV

   Available Since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-Gsdmd

   Plasmid

   #80950

   Purpose

   Expresses Gsdmd in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd (Gsdmd Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-BRD7

   Plasmid

   #65379

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   BRD7 (BRD7 Human)
   

   Tags

   EmGFP and V5

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9n(BB)-2A-GFP (PX461)

   Plasmid

   #48140

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-ZDHHC8

    Plasmid

    #231657

    Purpose

    Expresses human ZDHHC8 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC8 (ZDHHC8 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSKI

    Plasmid

    #232484

    Purpose

    pSKI can be used for single-copy transgene expression within the SKI PLACE System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.

    Depositor

    Carlos Silva García

    Article

    Dinneen et al G3 (Bethesda). 2025 Sep 19:jkaf220. doi:

    Insert

    pSKI
    

    Expression

    Worm

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTAT-CRE

    Plasmid

    #35619

    Depositor

    Steven Dowdy

    Article

    Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

    Insert

    CRE (cre Bacteriophage P1)
    

    Tags

    6xHIS, T7 tag, and TAT

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pCMVtight-UPRT-T2A-RFP-IRES-CD

    Plasmid

    #126677

    Purpose

    Doxycycline inducible expression of UPRT and CD along with reporter RFP

    Depositor

    Joan Massague

    Article

    Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

    Insert

    UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
    

    Promoter

    tight TRE

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-BCR-ABL-p210-FLAG

    Plasmid

    #205619

    Purpose

    Expression of BCR-ABL oncogene in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gregor et al Cell Mol Life Sci. 2020 Oct;77(19):3885-

    Insert

    BCR-ABL oncogene, p210 isoform b3a2
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-TetOne-Puro-hAXL

    Plasmid

    #124797

    Purpose

    Tet-inducible AXL expression in mammalian cells

    Depositor

    Kenneth Pienta

    Article

    Axelrod et al Mol Cancer Res. 2019 Feb;17(2):356-369.

    Insert

    AXL (AXL Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    June 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-ZDHHC14

    Plasmid

    #231662

    Purpose

    Expresses human ZDHHC14 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC14 (ZDHHC14 Human)
    

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Available Since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAcGFP-N1_CBP

    Plasmid

    #221903

    Purpose

    Transient expression of GFP-tagged CBP in mammalian cells

    Depositor

    Daniel Bose

    Insert

    CBP (CREBBP Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCSII-EF-miRFP670v1-hGem(1/110)

    Plasmid

    #80006

    Purpose

    hGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

    Insert

    hGeminin (1/110) (GMNN Human)
    

    Use

    Lentiviral

    Tags

    miRFP670v1

    Promoter

    EF-1 alpha

    Available Since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mCherry-Alpha-Actinin2-N-18

    Plasmid

    #54974

    Purpose

    Localization: Alpha Actinin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Alpha-Actinin2 (ACTN2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pAAV.CAG.GCaMP6s.WPRE.SV40

    Plasmid

    #100844

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge