165,888 results

1. pBABE puro PPAR delta

   Plasmid

   #8891

   Depositor

   Bruce Spiegelman

   Article

   Brun et al Genes Dev. 1996 Apr 15. 10(8):974-84.

   Insert

   PPAR delta (Ppard Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-HA-JMJD3

   Plasmid

   #24167

   Purpose

   Mammalian expression of human JMJD3 with HA tag

   Depositor

   Kristian Helin

   Article

   Agger et al Nature. 2007 Oct 11. 449(7163):731-4.

   Insert

   JMJD3 (KDM6B Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUBC-SV40NLS-PfV-Sapphire-IRES-H2B-mCherry

   Plasmid

   #203652

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a secondary translation initiation site for H2B-mCherry expression.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire-IRES-H2B-mCherry

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-Gsdmd-CT

   Plasmid

   #80953

   Purpose

   Expresses Gsdmd-CT in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd-CT (Gsdmd Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA-CasRx_SD1-pSV40-TagBFP

   Plasmid

   #221004

   Purpose

   Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

   Depositor

   Mikko Taipale, Benjamin Blencowe

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   CasRx 30nt processed direct repeat with SD1 mutation
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   DR1 A>T

   Promoter

   U6

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAV1-mCherry

   Plasmid

   #27705

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   caveolin-1 (CAV1 Canis lupus familiaris)
   

   Use

   Tags

   monomeric Cherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAB150

   Plasmid

   #101661

   Purpose

   Opto-T7RNAP*(563)

   Depositor

   Mustafa Khammash

   Article

   Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

   Insert

   Opto-T7RNAP*(563)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-SZT2

   Plasmid

   #87035

   Purpose

   mammalian expression of SZT2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   SZT2 (SZT2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1

   Plasmid

   #214994

   Purpose

   Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-TREG3G-PE2-rtTA3G-P2A-eGFP

    Plasmid

    #196971

    Purpose

    Piggybac vector with doxycyclin-inducible prime editor for mammalian cells

    Depositor

    Leopold Parts

    Inserts

    Cas9-RT
    rtTA3G-P2A-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK and TREG3G

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-PE2-P2A-GFP

    Plasmid

    #132776

    Purpose

    Prime editing in mammalian cells with co-translational GFP expression

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2-P2A-GFP
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTCF5 HLA-A*01:01

    Plasmid

    #180448

    Purpose

    E. coli expression of soluble MHC protein

    Depositor

    John Altman

    Insert

    HLA-A*01:01
    

    Use

    Tags

    BSP41

    Expression

    Bacterial

    Mutation

    Replacement of transmembrane domain with enzymati…

    Promoter

    Available Since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-MBP-huBsCas12a

    Plasmid

    #174671

    Purpose

    Expresses 6xHis-MBP tagged BsCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBsCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV, NLS and NLS, 3xHA, T2A, EGFP, 6x…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV 3xFLAG-GABARAP WT

    Plasmid

    #123097

    Purpose

    Expresses 3xFLAG-GABARAP wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMDC32-HPB

    Plasmid

    #32078

    Depositor

    Fumi Katagiri

    Article

    Qi et al Plant J. 2009 Mar;57(5):932-44. Epub 200

    Type

    Empty backbone

    Use

    Gateway destination binary vector

    Tags

    HA-Prescission-Biotin

    Expression

    Mutation

    Promoter

    35S promoter

    Available Since

    Dec. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. anxA1-GFP

    Plasmid

    #107194

    Purpose

    Expresses porcine annexin A1-GFP fusion protein for live cell imaging

    Depositor

    Ursula Rescher, Volker Gerke

    Article

    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

    Insert

    annexin A1
    

    Use

    Tags

    Green Fluorescent Protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3xnls-mScarlet-I

    Plasmid

    #98816

    Purpose

    In vivo visualization of the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    3xNLS
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-TO-TEVP

    Plasmid

    #116062

    Purpose

    Expresses TEV protease

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    TEVP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. psfaRb-Tn5

    Plasmid

    #183638

    Purpose

    Expresses anti-rabbit nanobody fused with Tn5

    Depositor

    Goncalo Castelo-Branco

    Article

    Bartosovic et al Nat Biotechnol. 2023 Jun;41(6):794-805.

    Insert

    anti rabbit secondary nanobody fused to Tn5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. G6PD/pRK5

    Plasmid

    #41521

    Depositor

    Xiaolu Yang

    Article

    Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

    Insert

    glucose-6-phosphate dehydrogenase (G6PD Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge