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Plasmid#23348PurposeExpresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.DepositorInsertPAGFP
UseTagsmitoExpressionMammalianMutationPromoterAvailable sinceApril 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)
Plasmid#181745Purposedual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1DepositorInserthuman/zebrafish codon optimized SpCas9
UseIn vitro transcription; t7 promoterTagsNLS(SV40)-3xFLAGExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD555 ZF150x6-Compact_EYFP
Plasmid#138869PurposeEYFP reporter vector for the ZF150x6-Compact promoterDepositorInsertZF150x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF150x6-CompactAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF0239
Plasmid#142385PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMYCL (MYCL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cre-P2A-dTomato (AAV1)
Viral Prep#107738-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mEos2
Plasmid#105128PurposeExpresses human FGAMS-mEos2 fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseTagsmEos2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ptet-RFP-shR-rtTA
Plasmid#35625PurposeTet-inducible AAV shRNA vector to test efficacy of shRNAs. Used in combination with pGFPns-reporter (Addgene plasmid #35626).DepositorTypeEmpty backboneUseAAV and RNAi; Tet inducibleTagsExpressionMammalianMutationPromoterPtetAvailable sinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP
Plasmid#176015PurposeMammalian expression of EGFP. Parton lab clone DFSDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
hSyn-BiPOLES-mCerulean
Plasmid#154944PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine
Plasmid#50472PurposeGs-coupled rM3D-IRES-mCitrine under the control of GFAP promoterDepositorUseAAVTagsHAExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV
Plasmid#72265PurposeExpress gene of interest under CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
eGFP L202 Reporter
Plasmid#119129PurposeActs as a reporter for APOBEC-medated single-base editing. mCherry acts as a transfection control and eGFP as a reporter.DepositorInsertmCherry and eGFP
UseLentiviralTagsExpressionMutationeGFP L202SPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD541 ZF13x6-Compact_EYFP
Plasmid#138855PurposeEYFP reporter vector for the ZF13x6-Compact promoterDepositorInsertZF13x6-Compact
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterZF13x6-CompactAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
plenti-FLAG-CAMK2D-ED
Plasmid#221700PurposeLentiviral expression of FLAG-tagged human CAMK2D (K43R/D136N) in mammalian cellsDepositorInsertCAMK2D (CAMK2D Human)
UseLentiviralTagsExpressionMutationK43R, D136NPromoterAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-GFP.W
Plasmid#51010PurposeLentivector that expresses LifeAct-GFP from internal human PGK promoterDepositorInsertLifeAct-GFP
UseLentiviralTagsLifeActExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
Red Glifon 3000
Plasmid#163116PurposeRed fluorescent protein-based glucose sensor for mammalian cells with lower affinityDepositorInsertRed Glifon 3000
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO EYFP
Plasmid#27056DepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, and AAV9InsertEYFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
iYFP-Parkin Plasmid
Plasmid#197107PurposeExpresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.DepositorInsertparkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
UseRetroviralTagsYFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only