161,670 results

1. pJL1 ATF1

   Plasmid

   #106286

   Purpose

   Expresses ATF1 in Ecoli off a T7 promoter

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   ATF1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYTK083

   Plasmid

   #65190

   Purpose

   Encodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK system

   Depositor

   John Dueber

   Article

   Lee et al ACS Synth Biol. 2015 Apr 14.

   Insert

   AmpR-ColE1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330S-2-PITCh

   Plasmid

   #63670

   Purpose

   Expresses Cas9 nuclease and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP2832

   Plasmid

   #160672

   Purpose

   Mobile CRISPRi sfGFP 'test', gmc6 sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   gmc6 (gfp) sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJMP2820

   Plasmid

   #160669

   Purpose

   Mobile CRISPRi sfGFP 'test', empty sgRNA

   Depositor

   Jason Peters

   Article

   Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

   Insert

   empty sgRNA (BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. UPB-duodenum (UPB6)

   Plasmid

   #180785

   Purpose

   Unique Projection Barcode (UPB6)-Duodenum for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM4D(Gi) (partial )
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-neo-hCG

   Plasmid

   #16574

   Depositor

   Bert Vogelstein

   Article

   Shih et al Nat Med. 2000 Jun . 6(6):711-4.

   Insert

   Beta-hCG (CGB3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pFREE_Zeo

   Plasmid

   #92053

   Purpose

   Allows inducible expression of gRNAs and Cas9 for plasmid curing.

   Depositor

   Morten Norholm

   Article

   Lauritsen et al Microb Cell Fact. 2017 Aug 2;16(1):135.

   Inserts

   Cas9
   gRNA array
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pRhamBAD and ptet

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-Pol-II 1-22

   Plasmid

   #35178

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al RNA. 2004 Apr;10(4):581-9.

   Insert

   RPBI (POLR2A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of C-terminal domain repeats 23-52

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pHTC-HaloTag_WDR91:1-747

    Plasmid

    #210997

    Purpose

    Mammalian expression of full-length human WDR91. C-terminal HaloTag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al bioRxiv 2024.03.03.583197

    Insert

    WDR91:M1-A747 (WDR91 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Feb. 21, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid

    #177868

    Purpose

    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d

    Insert

    mGreenLantern-based EXSISERS
    

    Use

    CRISPR, Synthetic Biology, and Unspecified

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMpGWB110

    Plasmid

    #68564

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-Pol-II 31-52

    Plasmid

    #35179

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al RNA. 2004 Apr;10(4):581-9.

    Insert

    RPBI (POLR2A Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain repeats 1-30

    Promoter

    CMV

    Available since

    Oct. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

14. mPlum-Rab11a-7

    Plasmid

    #55998

    Purpose

    Localization: Ras GTPase, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Tags

    mPlum

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pJMP2846

    Plasmid

    #160676

    Purpose

    Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

    Depositor

    Jason Peters

    Article

    Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBABE-neo-hTERT

    Plasmid

    #1774

    Purpose

    Retroviral expression of hTERT. Used to create immortalized cell lines.

    Depositor

    Bob Weinberg

    Article

    Counter et al Proc Natl Acad Sci U S A 1998 Dec 8;95(2

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEK19

    Plasmid

    #210578

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter
    

    Use

    Luciferase

    Tags

    NanoLuc luciferase

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    BdBIP1pro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST17-hOPTN

    Plasmid

    #23053

    Depositor

    Jon Ashwell

    Article

    Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    May 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC1GFP-Kir7.1

    Plasmid

    #79118

    Purpose

    Express N-terminal fused Kir7.1 protein in mammalian cells

    Depositor

    Bikash Pattnaik

    Article

    Pattnaik et al PLoS One. 2013 Aug 19;8(8):e71744. doi:

    Insert

    Inwardly rectifying potassium channel KCNJ13 (KCNJ13 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge