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pNW55
(Plasmid #22988)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22988 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescriptKS
  • Backbone size w/o insert (bp) 2964
  • Vector type
    Plant Expression, RNAi ; Plant gene inactivation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    osa-MIR528
  • Species
    O. sativa (rice)
  • Insert Size (bp)
    260

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SmaI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

See http://wmd3.weigelworld.org/

for more information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNW55 was a gift from Detlef Weigel (Addgene plasmid # 22988)
  • For your References section:

    Highly specific gene silencing by artificial miRNAs in rice. Warthmann N, Chen H, Ossowski S, Weigel D, Herve P. PLoS One. 2008 . 3(3):e1829. 10.1371/journal.pone.0001829 PubMed 18350165