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pRS300
(Plasmid #22846)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22846 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescriptSK+
  • Backbone size w/o insert (bp) 2964
  • Vector type
    Plant Expression, RNAi ; Plant gene inactivation

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MIR319a
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    404
  • Entrez Gene
    MIR319a (a.k.a. AT4G23713, JAGGED AND WAVY, JAW, MICRORNA 319, MIR319, MIR319A, microRNA319A, p_MI0000544)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma1 (destroyed during cloning)
  • 3′ cloning site Sma1 (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

See http://wmd3.weigelworld.org/

for more information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS300 was a gift from Detlef Weigel (Addgene plasmid # 22846)
  • For your References section:

    Highly specific gene silencing by artificial microRNAs in Arabidopsis. Schwab R, Ossowski S, Riester M, Warthmann N, Weigel D. Plant Cell. 2006 May . 18(5):1121-33. 10.1105/tpc.105.039834 PubMed 16531494