168,037 results

1. 20XUAS IVS CsChrimson tdtomato_tr

   Plasmid

   #111544

   Purpose

   Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

   Depositor

   Vivek Jayaraman

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chrimson_tdtomato_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB_r5-HT1.0

   Plasmid

   #208719

   Purpose

   Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

   Depositor

   Yulong Li

   Article

   Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

   Insert

   Red fluorescent 5-HT sensor GRAB_r5-HT1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAMD1-4

   Plasmid

   #61565

   Purpose

   Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

   Type

   Empty backbone

   Use

   Synthetic Biology; Mini-tn5 vector

   Available Since

   Jan. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Kl-EGFP

   Plasmid

   #45532

   Depositor

   Zipora Yablonka-Reuveni

   Article

   Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

   Insert

   alpha Klotho (Kl Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CaMKIIa-EGFP (AAV1)

   Viral Prep

   #50469-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   CaMKII

   Tags

   EGFP

   Available Since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

7. pPB_mU6_enh-gRNA_Puro-T2A-BFP

   Plasmid

   #235571

   Purpose

   Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   sgRNA backbone
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRVL-1

   Plasmid

   #104579

   Purpose

   Contains mouse kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.

   Depositor

   Daniel Christ

   Article

   Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi

   Insert

   Mouse CL kappa
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiCRISPRv2R

   Plasmid

   #167932

   Purpose

   lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmiCas9

    Plasmid

    #162758

    Purpose

    express miCas9

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    Contains Brex27 sequence range is 4958-5065

    Promoter

    CMV

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-LC3

    Plasmid

    #11546

    Depositor

    Karla Kirkegaard

    Article

    Jackson et al PLoS Biol. 2005 May . 3(5):e156.

    Insert

    LC3B (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. A2_uSFFV

    Plasmid

    #153415

    Purpose

    Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    A*02:01-P2A-human beta2 microglobulin (B2M Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA

    Plasmid

    #192481

    Purpose

    To express CasRX and a gRNA

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    CasRx
    

    Use

    AAV

    Mutation

    No

    Promoter

    U6/EFS

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-gfap

    Plasmid

    #82401

    Purpose

    Gateway-compatible zebrafish gfap promoter

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    gfap promoter
    

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-GFP

    Plasmid

    #160697

    Purpose

    Expresses GFP in mammalian cells

    Depositor

    Christine Mayr

    Article

    Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_VNp-DNAseI

    Plasmid

    #182409

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-DNase I (DNASE1 Bovine)
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221-FOS

    Plasmid

    #79511

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human FOS CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    FOS (FOS Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAX APP 751

    Plasmid

    #30138

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-EGFP

    Plasmid

    #50465

    Purpose

    EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    lac promoter / tac promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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