168,037 results

1. PGC-1 alpha promoter luciferase delta CRE

   Plasmid

   #8888

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter dCRE (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Site-directed mutagenesis to remove the CREB bind…

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMPP-mCherry-hTRIM21

   Plasmid

   #104972

   Purpose

   Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

   Depositor

   Leo James

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Tripartite Motif containing 21 (TRIM21 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Promoter

   SFFV

   Available Since

   Feb. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNL[NlucP/NFAT-RE/Hygro] Vector

   Plasmid

   #236929

   Purpose

   Express NFAT response element in luciferase reporter assay

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   NFAT
   

   Use

   Luciferase

   Tags

   NanoLuc (R)

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCK002 pME mCerulean

   Plasmid

   #195956

   Purpose

   Gateway pME fluorophore

   Depositor

   Alexa Burger, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mCerulean
   

   Use

   Other

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-Cys-SpyCatcher002

   Plasmid

   #102829

   Purpose

   Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Cys-SpyCatcher002
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Cysteine residue in N-terminal region

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. GST-RH▲-MNase

   Plasmid

   #136292

   Purpose

   Expression of catalytic inactive RNase H fused to micrococcal nuclease

   Depositor

   Kavitha Sarma

   Article

   Yan et al Cell Rep. 2019 Oct 29;29(5):1369-1380.e5

   Insert

   RNase HI-D10R-E48R and MNase
   

   Expression

   Bacterial

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMP1039

   Plasmid

   #119239

   Purpose

   expresses Tn7 transposase

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   tnsABCD
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ132B2.0

   Plasmid

   #99888

   Purpose

   Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU3

   Available Since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-41: ATP2A2-mEGFP

   Plasmid

   #114405

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

   Depositor

   Allen Institute for Cell Science

   Insert

   ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pSEP-LAMP1-RFP

    Plasmid

    #167970

    Purpose

    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

    Depositor

    Juan Bonifacino

    Article

    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    LAMP1 (Lamp1 Rat)
    

    Tags

    SEP and mRFP

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

    Plasmid

    #212963

    Purpose

    Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

    Depositor

    Roland Rad

    Article

    Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

    Insert

    DR1_CasRx_GFP targeting spacer
    

    Use

    CRISPR and Lentiviral

    Tags

    TagRFP657

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCrAb-km

    Plasmid

    #214199

    Purpose

    Expresses crRNA for genome editing in Acinetobacter baumannii

    Depositor

    Yu Wang

    Article

    Wang et al Curr Issues Mol Biol. 2024 Jan 5;46(1):5

    Insert

    crRNA-HDV
    

    Use

    CRISPR

    Promoter

    J23119

    Available Since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC1-HyPer-Red

    Plasmid

    #48249

    Purpose

    Genetically encoded hydrogen peroxide indicator with red fluorescence.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

    Insert

    HYPER-RED
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXTL-T7max-RLuc

    Plasmid

    #188312

    Purpose

    Expression of Renilla luciferase via a T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    Renilla luciferase
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2014-AaCas12b

    Plasmid

    #121949

    Purpose

    E. coli expression, protein purification

    Depositor

    Wei Li

    Article

    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

    Insert

    AaCas12b
    

    Tags

    10xHis tag

    Expression

    Bacterial

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pR26-CMVconst

    Plasmid

    #127373

    Purpose

    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Promoter

    CMV enhancer/promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

    Plasmid

    #159540

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G12C

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MARIO/ pcDNA3

    Plasmid

    #124838

    Purpose

    mammalian expression of MARIO, a genetically-encoded fluorescent Mg2+ sensor

    Depositor

    Takeharu Nagai

    Article

    Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

    Insert

    MARIO
    

    Tags

    No tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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