163,991 results

1. pTP1122

   Plasmid

   #104159

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEx-ER-TurboID

   Plasmid

   #160857

   Purpose

   Cre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   CAG

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Ursula Jakob, Michael Gray

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Use

   Tags

   6X His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAd/CMV/V5-Clasp2α

   Plasmid

   #89763

   Purpose

   Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

   Depositor

   Torsten Wittmann

   Article

   Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

   Insert

   CLASP2α (CLASP2 Human)
   

   Use

   Adenoviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCI H2B-RFP

   Plasmid

   #92398

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and IRES controlled Histone2B-mRFP1 reporter.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   IRES H2B RFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

   Plasmid

   #60229

   Purpose

   Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   sgRNA
   Cre recombinase
   

   Use

   AAV, CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   Cre-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and U6

   Available since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

   Plasmid

   #185916

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   human codon optimized ABE8.20m-nSpG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC13N-iCAG.copGFP

   Plasmid

   #66578

   Purpose

   CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

   Depositor

   Jizhong Zou

   Article

   Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

   Insert

   copGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_BE4max_P2A_GFP

   Plasmid

   #112099

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   BE4max_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AiP12123 - pAAV-AiE0397m-minBglobin-SYFP2-WPRE3-BGHpA (Alias: CN2123)

    Plasmid

    #208150

    Purpose

    Direct-expressing SYFP2 in oligodendrocytes AAV Virus

    Depositor

    The Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    Beta Globin minimal promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

    Plasmid

    #131004

    Purpose

    bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    Kv2.1-HA

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-ExRai-AMPKAR

    Plasmid

    #192446

    Purpose

    Genetically encoded excitation-ratiometric fluorescent biosensor for monitoring AMPK activity in living cells.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    ExRai-AMPKAR
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-LC3 (human)

    Plasmid

    #24920

    Purpose

    Mammalian expression of LC3 fused to EGFP

    Depositor

    Toren Finkel

    Article

    Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

    Insert

    LC3 (MAP1LC3B Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENN.AAV.CamKII 0.4.Cre.SV40 (AAV5)

    Viral Prep

    #105558-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pENN.AAV.CamKII 0.4.Cre.SV40 (#105558). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII 0.4.Cre.SV40 plasmid DNA. CamKII-driven Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    CaMKII

    Tags

    None

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-NCad-GA

    Plasmid

    #191666

    Purpose

    ddGFP-A-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddGFP-A

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (AAV9)

    Viral Prep

    #100838-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 (#100838). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Tags

    None

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

17. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-NICD

    Plasmid

    #26891

    Depositor

    Nicholas Gaiano

    Article

    Dang et al Dev Neurosci. 2006;28(1-2):58-69.

    Insert

    NICD (Notch1 Mouse)
    

    Use

    Tags

    FLAG and b-globin polyA

    Expression

    Mammalian

    Mutation

    E2299K

    Promoter

    Available since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

19. AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51087

    Purpose

    Forebrain principle neuron specific expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa 1.3 kb

    Available since

    Feb. 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. 6xHis-SNAP-PI(3)P Probe

    Plasmid

    #211508

    Purpose

    For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

    Depositor

    David Murray, Hannes Maib

    Article

    Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

    Insert

    FYVE-Hrs x 2 (PIKFYVE Human)
    

    Use

    Tags

    6xhis-SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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