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pHSN6A01
(Plasmid #50586)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50586 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreen-like binary vector
  • Backbone manufacturer
    Mullineaux Lab, see http://www.pgreen.ac.uk/
  • Vector type
    CRISPR ; Plant expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    dCas9-VP64
  • Alt name
    Cas9
  • Species
    Synthetic
  • Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16 activation domain; maize codon–optimized dCas9-VP64 vectors were designed for targeted activation of the genes of interest
  • Promoter 2×35Sp
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)
    • HA (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    gRNA scaffold
  • Species
    Synthetic
  • Promoter AtU6-26p

Cloning Information for Gene/Insert 2

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHSN6A01 was a gift from Qi-Jun Chen (Addgene plasmid # 50586 ; http://n2t.net/addgene:50586 ; RRID:Addgene_50586)
  • For your References section:

    A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517