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pHSN501
(Plasmid #50589)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 50589 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreen-like binary vector
  • Backbone manufacturer
    Mullineaux Lab, see http://www.pgreen.ac.uk/
  • Vector type
    CRISPR ; Plant expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin and Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    zCas9D10A
  • Alt name
    Cas9
  • Species
    Synthetic
  • Mutation
    Cas9D10A nickase, was derived from the zCas9 and included in the toolkit to facilitate HR-based gene replacement or NHEJ-based insertion/deletion events (indels) through offset nicking under the precondition of the avoidance of off-target effects
  • Promoter 2×35Sp
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • NLS (N terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    gRNA scaffold
  • Species
    Synthetic
  • Promoter AtU6-26p

Cloning Information for Gene/Insert 2

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHSN501 was a gift from Qi-Jun Chen (Addgene plasmid # 50589 ; http://n2t.net/addgene:50589 ; RRID:Addgene_50589)
  • For your References section:

    A CRISPR/Cas9 toolkit for multiplex genome editing in plants. Xing HL, Dong L, Wang ZP, Zhang HY, Han CY, Liu B, Wang XC, Chen QJ. BMC Plant Biol. 2014 Nov 29;14(1):327. 10.1186/s12870-014-0327-y PubMed 25432517