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(Plasmid #69862)


Item Catalog # Description Quantity Price (USD)
Plasmid 69862 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Modifications to backbone
    URA3 gene was introduced into pCAP01 under the strong promoter of the Schizosaccharomyces pombe ADH1 gene (pADH1) into the SpeI and KpnI restriction sites of pCAP01, as a counter selectable marker, to produce pCAP03. pADH1 can tolerate an insertion of up to 130 bp between the TATA box and the transcription initiation site. This method allows the use of shorter capture arms and conveniently selects against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA). In order to easy linearize pCAP03, the 1369 bp apramycin resistance cassette (acc(3)IV) was amplified from pIJ773 and cloned into the pCAP03 to obtain pCAP03-acc(3)IV .
  • Vector type
    Bacterial Expression, Yeast Expression
  • Selectable markers
    TRP1, URA3 ; apramycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    The vector also contain the apramycin resistance gene from pIJ773
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Noskov, V. N., Kouprina, N., Leem, S. H., Ouspenski, I., Barrett, J. C., and Larionov, V. (2003) A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast, BMC Genomics 4, 16.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAP03-acc(3)IV was a gift from Bradley Moore (Addgene plasmid # 69862 ; ; RRID:Addgene_69862)
  • For your References section:

    Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining. Tang X, Li J, Millan-Aguinaga N, Zhang JJ, O'Neill EC, Ugalde JA, Jensen PR, Mantovani SM, Moore BS. ACS Chem Biol. 2015 Oct 21. 10.1021/acschembio.5b00658 PubMed 26458099