Purpose(Empty Backbone) The function of this material is to facilitate the capture and expression of bacterial gene clusters.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59981||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size (bp) 7600
Modifications to backboneThe RP4 origin and attP site was inserted into the vector for heterologous expressing biosynthetic gene cluster in actinobacteria. The vector also contains the TRP1 gene and CEN6_ARS4 for selecting in S. cerevisiae tryptophan deficient mutant.
Vector typeBacterial Expression, Yeast Expression, Synthetic Biology ; transformation-associated recombination cloning
- Promoter no
Selectable markersTRP1, URA3
/ Fusion Protein
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer TATGTCCTGCGGGTAAATAG
- 3′ sequencing primer TCGGGGAAATGTGCGCGGAA (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAP01 was a gift from Bradley Moore (Addgene plasmid # 59981 ; http://n2t.net/addgene:59981 ; RRID:Addgene_59981)
For your References section:Direct cloning and refactoring of a silent lipopeptide biosynthetic gene cluster yields the antibiotic taromycin A. Yamanaka K, Reynolds KA, Kersten RD, Ryan KS, Gonzalez DJ, Nizet V, Dorrestein PC, Moore BS. Proc Natl Acad Sci U S A. 2014 Feb 4;111(5):1957-62. doi: 10.1073/pnas.1319584111. Epub 2014 Jan 21. 10.1073/pnas.1319584111 PubMed 24449899